Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript… Continue reading
Tag: vector
Engineering hybrid genes without the use of restriction enzymes: gene splicing
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene splicing by overlap extension is a new approach for recombining DNA molecules at precise junctions irrespective of nucleotide sequences at the recombination site and without the use of restriction endonucleases or ligase. Fragments from the genes that are to be… Continue reading
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. A recent cluster of pneumonia cases in Wuhan, China, was caused by a novel betacoronavirus, the 2019 novel coronavirus (2019-nCoV). We report the epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of these patients.All patients with suspected 2019-nCoV were admitted… Continue reading
Supervised risk predictor of breast cancer based on intrinsic subtypes.
Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains… Continue reading
Primer3–new capabilities and interfaces.
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Accurate normalization is an absolute prerequisite for correct measurement of gene expression. For quantitative real-time reverse transcription-PCR (RT-PCR), the most commonly used normalization strategy involves standardization to a single constitutively expressed… Continue reading