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Primer3–new capabilities and interfaces.

Posted on April 29, 2021 by Leah

Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.

Accurate normalization is an absolute prerequisite for correct measurement of gene expression. For quantitative real-time reverse transcription-PCR (RT-PCR), the most commonly used normalization strategy involves standardization to a single constitutively expressed control gene. However, in recent years, it has become clear that no single gene is constitutively expressed in all cell types and under all experimental conditions, implying that the expression stability of the intended control gene has to be verified before each experiment. We outline a novel, innovative, and robust strategy to identify stably expressed genes among a set of candidate normalization genes. The strategy is rooted in a mathematical model of gene expression that enables estimation not only of the overall variation of the candidate normalization genes but also of the variation between sample subgroups of the sample set.
Notably, the strategy provides a direct measure for the estimated expression variation, enabling the user to evaluate the systematic error introduced when using the gene. In a side-by-side comparison with a previously published strategy, our model-based approach performed in a more robust manner and showed less sensitivity toward coregulation of the candidate normalization genes. We used the model-based strategy to identify genes suited to normalize quantitative RT-PCR data from colon cancer and bladder cancer. These genes are UBC, GAPD, and TPT1 for the colon and HSPCB, TEGT, and ATP5B for the bladder. The presented strategy can be applied to evaluate the suitability of any normalization gene candidate in any kind of experimental design and should allow more reliable normalization of RT-PCR data.
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inqua-saccom

DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

We describe “universal” DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.

Real-time quantification of microRNAs by stem-loop RT-PCR.

A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide. Furthermore, they are not affected by genomic DNA contamination. Precise quantification is achieved routinely with as little as 25 pg of total RNA for most miRNAs.
In fact, the high sensitivity, specificity and precision of this method allows for direct analysis of a single cell without nucleic acid purification. Like standard TaqMan gene expression assays, TaqMan miRNA assays exhibit a dynamic range of seven orders of magnitude. Quantification of five miRNAs in seven mouse tissues showed variation from less than 10 to more than 30,000 copies per cell. This method enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases. Stem-loop RT-PCR can be used for the quantification of other small RNA molecules such as short interfering RNAs (siRNAs). Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency.
 

Primer3–new capabilities and interfaces.

Polymerase chain reaction (PCR) is a basic molecular biology technique with a multiplicity of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis of genes, diagnosis of diseases, genotyping and discovery of genetic variants. Reliable primer design is crucial for successful PCR, and for over a decade, the open-source Primer3 software has been widely used for primer design, often in high-throughput genomics applications.
It has also been incorporated into numerous publicly available software packages and web services. During this period, we have greatly expanded Primer3’s functionality. In this article, we describe Primer3’s current capabilities, emphasizing recent improvements. The most notable enhancements incorporate more accurate thermodynamic models in the primer design process, both to improve melting temperature prediction and to reduce the likelihood that primers will form hairpins or dimers.
Additional enhancements include more precise control of primer placement-a change motivated partly by opportunities to use whole-genome sequences to improve primer specificity. We also added features to increase ease of use, including the ability to save and re-use parameter settings and the ability to require that individual primers not be used in more than one primer pair. We have made the core code more modular and provided cleaner programming interfaces to further ease integration with other software. These improvements position Primer3 for continued use with genome-scale data in the decade ahead.

Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

Precise mapping of DNA methylation patterns in CpG islands has become essential for understanding diverse biological processes such as the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in human cancer. We describe a new method, MSP (methylation-specific PCR), which can rapidly assess the methylation status of virtually any group of CpG sites within a CpG island, independent of the use of methylation-sensitive restriction enzymes.
This assay entails initial modification of DNA by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA. MSP requires only small quantities of DNA, is sensitive to 0.1% methylated alleles of a given CpG island locus, and can be performed on DNA extracted from paraffin-embedded samples.
MSP eliminates the false positive results inherent to previous PCR-based approaches which relied on differential restriction enzyme cleavage to distinguish methylated from unmethylated DNA. In this study, we demonstrate the use of MSP to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin, and von Hippel-Lindau) in human cancer.

Alpha-bungarotoxin, CF568

9-00006 Biotium
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007 Biotium
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007 Biotium
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009 Biotium
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009 Biotium
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 Biotium 1MG
EUR 168
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 Biotium 500uG
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 Biotium 500uG
EUR 383
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 Biotium 10ST
EUR 417
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 Biotium 10ST
EUR 466
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 Biotium 500uG
EUR 466
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 Biotium 10ST
EUR 523
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 Biotium 500uG
EUR 433
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018 Biotium
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018 Biotium
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 Biotium 1MG
EUR 314
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 Biotium 20ST
EUR 418
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 Biotium 1MG
EUR 344
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 Biotium 20ST
EUR 448
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 Biotium 1MG
EUR 491
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 Biotium 20ST
EUR 625
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 Biotium 1MG
EUR 281
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 Biotium 100uG
EUR 139
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 Biotium 500uG
EUR 494
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 Biotium 300
EUR 345
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 Biotium 100U
EUR 428
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 Biotium 300U
EUR 345
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 Biotium 300ST
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033 Biotium 300EU
EUR 345
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036 Biotium 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037 Biotium 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A

00042-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543

00043 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543

00043-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568

00044 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568

00044-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594

00045 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594

00045-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633

00046 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633

00046-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R

00047 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R

00047-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R

00048 Biotium 300U
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R

00048-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350

00049 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350

00049-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R

00050 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R

00050-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532

00051 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532

00051-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C

00052 Biotium 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C

00052-T Biotium 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680

00053 Biotium 300U
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680

00053-T Biotium 50U
EUR 147
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF430

00054 Biotium 300U
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF430

00054-T Biotium 50U
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF440

00055 Biotium 300U
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF440

00055-T Biotium 50U
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Cholera Toxin Subunit B, CF488A conjugate

00070 Biotium 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF568 conjugate

00071 Biotium 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF594 conjugate

00072 Biotium 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF640R conjugate

00073 Biotium 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF532 conjugate

00074 Biotium 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF543 conjugate

00075 Biotium 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF620R conjugate

00076 Biotium 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF633 conjugate

00077 Biotium 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF660R conjugate

00078 Biotium 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF680R conjugate

00079 Biotium 100ug
EUR 282
Description: Minimum order quantity: 1 unit of 100ug

Human Transferrin, CF488A conjugate

00081 Biotium 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF543 conjugate

00082 Biotium 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF568 conjugate

00083 Biotium 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF594 conjugate

00084 Biotium 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF640R conjugate

00085 Biotium 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF680R conjugate

00086 Biotium 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF750 conjugate

00087 Biotium 1mg
EUR 155
Description: Minimum order quantity: 1 unit of 1mg

HIV-1 tat recombinant antigen

00110-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HIV-1 tat recombinant antigen full length 15 kDa

HIV-1 tat recombinant antigen

00110-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HIV-1 tat recombinant antigen full length 15 kDa

HIV-1 GAG P24 Recombinant Antigen

00111-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa

HIV-1 GAG P24 Recombinant Antigen

00111-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa

HIV-1 nef recombinant antigen

00112-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa

HIV-1 nef recombinant antigen

00112-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa

HIV-1 env gp41 recombinant antigen

00113-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa

HIV-1 env gp41 recombinant antigen

00113-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa

HIV-2 GP 36 Recombinant Antigen

00114-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa

HIV-2 GP 36 Recombinant Antigen

00114-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa

HCV core recombinant antigen

00115-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa

HCV core recombinant antigen

00115-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa

HCV core recombinant antigen, Biotin conjugate

00115-V-B-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate.

HCV core recombinant antigen, Biotin conjugate

00115-V-B-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate.

HCV core recombinant antigen, FITC conjugate

00115-V-F-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate.

HCV core recombinant antigen, FITC conjugate

00115-V-F-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate.

HCV core recombinant antigen, Rhodamine

00115-V-R-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

HCV core recombinant antigen, Rhodamine

00115-V-R-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b

00116-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa.

HCV NS4 recombinant antigen NS4a+b

00116-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa.

HCV NS4 recombinant antigen NS4a+b, Biotin

00116-V-B-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate.

HCV NS4 recombinant antigen NS4a+b, Biotin

00116-V-B-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate.

HCV NS4 recombinant antigen NS4a+b, FITC

00116-V-F-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate.

HCV NS4 recombinant antigen NS4a+b, FITC

00116-V-F-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine

00116-V-R-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine

00116-V-R-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS3 recombinant antigen

00117-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa

HCV NS3 recombinant antigen

00117-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa

HBV core recombinant antigen

00120-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa

HBV core recombinant antigen

00120-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa

HBV core recombinant antigen, Delta

00121-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa

HBV core recombinant antigen, Delta

00121-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa

HBV surface recombinant antigen HBsAg

00122-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant

HBV surface recombinant antigen HBsAg

00122-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant

HBV surface recombinant antigen HBsAg antigen

00123-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity

HBV surface recombinant antigen HBsAg antigen

00123-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity

HBV surface recombinant antigen HBsAg antigen

00124-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant

HBV surface recombinant antigen HBsAg antigen

00124-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant

HEV (Birma) ORF2 recombinant antigen

00131-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659.

HEV (Birma) ORF2 recombinant antigen

00131-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659.

HEV (Birma) ORF2 recombinant antigen

00132-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461.

HEV (Birma) ORF2 recombinant antigen

00132-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461.

AA 403-461 HEV Birma ORF2 Recombinant Antigen

00133-V-01mg Virogen 0,1 mg
EUR 267.5
Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123.

AA 403-461 HEV Birma ORF2 Recombinant Antigen

00133-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123.

T, Pallidum recombinant antigen p15

00141-V-01mg Virogen 0,1 mg
EUR 267.5
Description: T. Pallidum recombinant antigen p15 (full length)

T, Pallidum recombinant antigen p15

00141-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: T. Pallidum recombinant antigen p15 (full length)

T, Pallidum recombinant antigen p17

00142-V-01mg Virogen 0,1 mg
EUR 267.5
Description: T. Pallidum recombinant antigen p17 (full length)

T, Pallidum recombinant antigen p17

00142-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: T. Pallidum recombinant antigen p17 (full length)

T, Pallidum recombinant antigen p45

00143-V-01mg Virogen 0,1 mg
EUR 267.5
Description: T. Pallidum recombinant antigen p45 (full length)

T, Pallidum recombinant antigen p45

00143-V-1000ug Virogen 1000 ug
EUR 1282.5
Description: T. Pallidum recombinant antigen p45 (full length)

T, pallidum recombinant antigen TmpA

00144-V-01mg Virogen 0,1 mg
EUR 267.5
Description: T. Pallidum recombinant antigen TmpA (full length)

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