Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.
Accurate normalization is an absolute prerequisite for correct measurement of gene expression. For quantitative real-time reverse transcription-PCR (RT-PCR), the most commonly used normalization strategy involves standardization to a single constitutively expressed control gene. However, in recent years, it has become clear that no single gene is constitutively expressed in all cell types and under all experimental conditions, implying that the expression stability of the intended control gene has to be verified before each experiment. We outline a novel, innovative, and robust strategy to identify stably expressed genes among a set of candidate normalization genes. The strategy is rooted in a mathematical model of gene expression that enables estimation not only of the overall variation of the candidate normalization genes but also of the variation between sample subgroups of the sample set.
Notably, the strategy provides a direct measure for the estimated expression variation, enabling the user to evaluate the systematic error introduced when using the gene. In a side-by-side comparison with a previously published strategy, our model-based approach performed in a more robust manner and showed less sensitivity toward coregulation of the candidate normalization genes. We used the model-based strategy to identify genes suited to normalize quantitative RT-PCR data from colon cancer and bladder cancer. These genes are UBC, GAPD, and TPT1 for the colon and HSPCB, TEGT, and ATP5B for the bladder. The presented strategy can be applied to evaluate the suitability of any normalization gene candidate in any kind of experimental design and should allow more reliable normalization of RT-PCR data.

DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.
We describe “universal” DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.
Real-time quantification of microRNAs by stem-loop RT-PCR.
A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide. Furthermore, they are not affected by genomic DNA contamination. Precise quantification is achieved routinely with as little as 25 pg of total RNA for most miRNAs.
In fact, the high sensitivity, specificity and precision of this method allows for direct analysis of a single cell without nucleic acid purification. Like standard TaqMan gene expression assays, TaqMan miRNA assays exhibit a dynamic range of seven orders of magnitude. Quantification of five miRNAs in seven mouse tissues showed variation from less than 10 to more than 30,000 copies per cell. This method enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases. Stem-loop RT-PCR can be used for the quantification of other small RNA molecules such as short interfering RNAs (siRNAs). Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency.
Primer3–new capabilities and interfaces.
Polymerase chain reaction (PCR) is a basic molecular biology technique with a multiplicity of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis of genes, diagnosis of diseases, genotyping and discovery of genetic variants. Reliable primer design is crucial for successful PCR, and for over a decade, the open-source Primer3 software has been widely used for primer design, often in high-throughput genomics applications.
It has also been incorporated into numerous publicly available software packages and web services. During this period, we have greatly expanded Primer3’s functionality. In this article, we describe Primer3’s current capabilities, emphasizing recent improvements. The most notable enhancements incorporate more accurate thermodynamic models in the primer design process, both to improve melting temperature prediction and to reduce the likelihood that primers will form hairpins or dimers.
Additional enhancements include more precise control of primer placement-a change motivated partly by opportunities to use whole-genome sequences to improve primer specificity. We also added features to increase ease of use, including the ability to save and re-use parameter settings and the ability to require that individual primers not be used in more than one primer pair. We have made the core code more modular and provided cleaner programming interfaces to further ease integration with other software. These improvements position Primer3 for continued use with genome-scale data in the decade ahead.
Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.
Precise mapping of DNA methylation patterns in CpG islands has become essential for understanding diverse biological processes such as the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in human cancer. We describe a new method, MSP (methylation-specific PCR), which can rapidly assess the methylation status of virtually any group of CpG sites within a CpG island, independent of the use of methylation-sensitive restriction enzymes.
This assay entails initial modification of DNA by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA. MSP requires only small quantities of DNA, is sensitive to 0.1% methylated alleles of a given CpG island locus, and can be performed on DNA extracted from paraffin-embedded samples.
MSP eliminates the false positive results inherent to previous PCR-based approaches which relied on differential restriction enzyme cleavage to distinguish methylated from unmethylated DNA. In this study, we demonstrate the use of MSP to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin, and von Hippel-Lindau) in human cancer.
Alpha-bungarotoxin, CF568 |
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9-00006 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
Alpha-bungarotoxin, CF594 |
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9-00007 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
Alpha-bungarotoxin CF594 |
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9-00007 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
Alpha-bungarotoxin, CF633 |
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9-00009 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
Alpha-bungarotoxin, CF633 |
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9-00009 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
Alpha-bungarotoxin |
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00010-1 | Biotium | 1MG | EUR 168 |
Description: Minimum order quantity: 1 unit of 1MG |
Fluorescein-Alpha-bungarotoxin |
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00011 | Biotium | 500uG | EUR 367 |
Description: Minimum order quantity: 1 unit of 500uG |
Tetramethylrhodamine-Alpha-bungarotoxin |
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00012 | Biotium | 500uG | EUR 383 |
Description: Minimum order quantity: 1 unit of 500uG |
Fluorescein-alpha-bungarotoxin: (10x50ug) |
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00013 | Biotium | 10ST | EUR 417 |
Description: Minimum order quantity: 1 unit of 10ST |
Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug) |
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00014 | Biotium | 10ST | EUR 466 |
Description: Minimum order quantity: 1 unit of 10ST |
Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug) |
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00015 | Biotium | 500uG | EUR 466 |
Description: Minimum order quantity: 1 unit of 500uG |
Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug) |
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00016 | Biotium | 10ST | EUR 523 |
Description: Minimum order quantity: 1 unit of 10ST |
Biotin-xx-a-bungarotoxin |
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00017 | Biotium | 500uG | EUR 433 |
Description: Minimum order quantity: 1 unit of 500uG |
Alpha-Bungarotoxin, CF555 conjugate |
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9-00018 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
Alpha-Bungarotoxin, CF555 conjugate |
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9-00018 | Biotium |
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Description: Minimum order quantity: 1 unit of 100ug |
Biotin-cAMP, diisopropylethylammonium salt: |
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00020 | Biotium | 1MG | EUR 314 |
Description: Minimum order quantity: 1 unit of 1MG |
Biotin-cAMP, diisopropylethylammonium salt: (20x50ug) |
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00020-1 | Biotium | 20ST | EUR 418 |
Description: Minimum order quantity: 1 unit of 20ST |
Biotin-cGMP, diisopropylethylammonium salt: |
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00021 | Biotium | 1MG | EUR 344 |
Description: Minimum order quantity: 1 unit of 1MG |
Biotin-cGMP, diisopropylethylammonium salt: (20x50ug) |
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00021-1 | Biotium | 20ST | EUR 448 |
Description: Minimum order quantity: 1 unit of 20ST |
Cyanine 644-cAMP: |
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00022 | Biotium | 1MG | EUR 491 |
Description: Minimum order quantity: 1 unit of 1MG |
Cyanine 644-cAMP: (20x50ug) |
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00022-1 | Biotium | 20ST | EUR 625 |
Description: Minimum order quantity: 1 unit of 20ST |
Fluorescein Methotrexate, triammonium salt: |
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00023 | Biotium | 1MG | EUR 281 |
Description: Minimum order quantity: 1 unit of 1MG |
Staurosporine |
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00025 | Biotium | 100uG | EUR 139 |
Description: Minimum order quantity: 1 unit of 100uG |
Alpha-bungarotoxin, CF543 |
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00026 | Biotium | 500uG | EUR 494 |
Description: Minimum order quantity: 1 unit of 500uG |
Rhodamine phalloidin 300u |
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00027 | Biotium | 300 | EUR 345 |
Description: Minimum order quantity: 1 unit of 300 |
Biotin-xx-phalloidin |
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00028 | Biotium | 100U | EUR 428 |
Description: Minimum order quantity: 1 unit of 100U |
Fluorescein-phalloidin |
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00030 | Biotium | 300U | EUR 345 |
Description: Minimum order quantity: 1 unit of 300U |
Rhodamine 110 phalloidin |
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00032 | Biotium | 300ST | EUR 345 |
Description: Minimum order quantity: 1 unit of 300ST |
Sulforhodamine 101 (Texas-Red) Phalloidin |
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00033 | Biotium | 300EU | EUR 345 |
Description: Minimum order quantity: 1 unit of 300EU |
Phalloidin, CF405M |
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00034 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF405M |
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00034-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
CF488A-cAMP |
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00036 | Biotium | 100ug | EUR 172 |
Description: Minimum order quantity: 1 unit of 100ug |
CF640R-cAMP |
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00037 | Biotium | 100ug | EUR 172 |
Description: Minimum order quantity: 1 unit of 100ug |
Phalloidin, CF555 |
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00040 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF555 |
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00040-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF647 |
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00041 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF647 |
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00041-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF488A |
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00042 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF488A |
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00042-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF543 |
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00043 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF543 |
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00043-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF568 |
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00044 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF568 |
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00044-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF594 |
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00045 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF594 |
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00045-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF633 |
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00046 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF633 |
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00046-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF660R |
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00047 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF660R |
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00047-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF680R |
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00048 | Biotium | 300U | EUR 466 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF680R |
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00048-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF350 |
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00049 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF350 |
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00049-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF640R |
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00050 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF640R |
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00050-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF532 |
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00051 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF532 |
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00051-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF660C |
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00052 | Biotium | 300U | EUR 442 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF660C |
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00052-T | Biotium | 50U | EUR 133 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF680 |
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00053 | Biotium | 300U | EUR 466 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF680 |
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00053-T | Biotium | 50U | EUR 147 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF430 |
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00054 | Biotium | 300U | EUR 449 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF430 |
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00054-T | Biotium | 50U | EUR 139 |
Description: Minimum order quantity: 1 unit of 50U |
Phalloidin, CF440 |
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00055 | Biotium | 300U | EUR 449 |
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF440 |
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00055-T | Biotium | 50U | EUR 139 |
Description: Minimum order quantity: 1 unit of 50U |
Cholera Toxin Subunit B, CF488A conjugate |
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00070 | Biotium | 100ug | EUR 261 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF568 conjugate |
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00071 | Biotium | 100ug | EUR 261 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF594 conjugate |
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00072 | Biotium | 100ug | EUR 261 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF640R conjugate |
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00073 | Biotium | 100ug | EUR 264 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF532 conjugate |
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00074 | Biotium | 100ug | EUR 264 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF543 conjugate |
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00075 | Biotium | 100ug | EUR 264 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF620R conjugate |
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00076 | Biotium | 100ug | EUR 264 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF633 conjugate |
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00077 | Biotium | 100ug | EUR 264 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF660R conjugate |
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00078 | Biotium | 100ug | EUR 264 |
Description: Minimum order quantity: 1 unit of 100ug |
Cholera Toxin Subunit B, CF680R conjugate |
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00079 | Biotium | 100ug | EUR 282 |
Description: Minimum order quantity: 1 unit of 100ug |
Human Transferrin, CF488A conjugate |
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00081 | Biotium | 1mg | EUR 149 |
Description: Minimum order quantity: 1 unit of 1mg |
Human Transferrin, CF543 conjugate |
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00082 | Biotium | 1mg | EUR 149 |
Description: Minimum order quantity: 1 unit of 1mg |
Human Transferrin, CF568 conjugate |
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00083 | Biotium | 1mg | EUR 149 |
Description: Minimum order quantity: 1 unit of 1mg |
Human Transferrin, CF594 conjugate |
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00084 | Biotium | 1mg | EUR 149 |
Description: Minimum order quantity: 1 unit of 1mg |
Human Transferrin, CF640R conjugate |
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00085 | Biotium | 1mg | EUR 149 |
Description: Minimum order quantity: 1 unit of 1mg |
Human Transferrin, CF680R conjugate |
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00086 | Biotium | 1mg | EUR 149 |
Description: Minimum order quantity: 1 unit of 1mg |
Human Transferrin, CF750 conjugate |
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00087 | Biotium | 1mg | EUR 155 |
Description: Minimum order quantity: 1 unit of 1mg |
HIV-1 tat recombinant antigen |
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00110-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HIV-1 tat recombinant antigen full length 15 kDa |
HIV-1 tat recombinant antigen |
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00110-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HIV-1 tat recombinant antigen full length 15 kDa |
HIV-1 GAG P24 Recombinant Antigen |
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00111-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa |
HIV-1 GAG P24 Recombinant Antigen |
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00111-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa |
HIV-1 nef recombinant antigen |
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00112-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa |
HIV-1 nef recombinant antigen |
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00112-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa |
HIV-1 env gp41 recombinant antigen |
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00113-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa |
HIV-1 env gp41 recombinant antigen |
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00113-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa |
HIV-2 GP 36 Recombinant Antigen |
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00114-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa |
HIV-2 GP 36 Recombinant Antigen |
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00114-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa |
HCV core recombinant antigen |
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00115-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa |
HCV core recombinant antigen |
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00115-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa |
HCV core recombinant antigen, Biotin conjugate |
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00115-V-B-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate. |
HCV core recombinant antigen, Biotin conjugate |
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00115-V-B-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate. |
HCV core recombinant antigen, FITC conjugate |
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00115-V-F-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate. |
HCV core recombinant antigen, FITC conjugate |
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00115-V-F-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate. |
HCV core recombinant antigen, Rhodamine |
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00115-V-R-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate. |
HCV core recombinant antigen, Rhodamine |
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00115-V-R-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate. |
HCV NS4 recombinant antigen NS4a+b |
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00116-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. |
HCV NS4 recombinant antigen NS4a+b |
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00116-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. |
HCV NS4 recombinant antigen NS4a+b, Biotin |
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00116-V-B-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate. |
HCV NS4 recombinant antigen NS4a+b, Biotin |
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00116-V-B-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate. |
HCV NS4 recombinant antigen NS4a+b, FITC |
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00116-V-F-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate. |
HCV NS4 recombinant antigen NS4a+b, FITC |
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00116-V-F-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate. |
HCV NS4 recombinant antigen NS4a+b, Rhodamine |
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00116-V-R-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate. |
HCV NS4 recombinant antigen NS4a+b, Rhodamine |
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00116-V-R-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate. |
HCV NS3 recombinant antigen |
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00117-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa |
HCV NS3 recombinant antigen |
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00117-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa |
HBV core recombinant antigen |
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00120-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa |
HBV core recombinant antigen |
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00120-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa |
HBV core recombinant antigen, Delta |
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00121-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa |
HBV core recombinant antigen, Delta |
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00121-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa |
HBV surface recombinant antigen HBsAg |
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00122-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant |
HBV surface recombinant antigen HBsAg |
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00122-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant |
HBV surface recombinant antigen HBsAg antigen |
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00123-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity |
HBV surface recombinant antigen HBsAg antigen |
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00123-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity |
HBV surface recombinant antigen HBsAg antigen |
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00124-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant |
HBV surface recombinant antigen HBsAg antigen |
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00124-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant |
HEV (Birma) ORF2 recombinant antigen |
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00131-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659. |
HEV (Birma) ORF2 recombinant antigen |
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00131-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659. |
HEV (Birma) ORF2 recombinant antigen |
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00132-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461. |
HEV (Birma) ORF2 recombinant antigen |
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00132-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461. |
AA 403-461 HEV Birma ORF2 Recombinant Antigen |
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00133-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123. |
AA 403-461 HEV Birma ORF2 Recombinant Antigen |
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00133-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123. |
T, Pallidum recombinant antigen p15 |
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00141-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: T. Pallidum recombinant antigen p15 (full length) |
T, Pallidum recombinant antigen p15 |
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00141-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: T. Pallidum recombinant antigen p15 (full length) |
T, Pallidum recombinant antigen p17 |
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00142-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: T. Pallidum recombinant antigen p17 (full length) |
T, Pallidum recombinant antigen p17 |
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00142-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: T. Pallidum recombinant antigen p17 (full length) |
T, Pallidum recombinant antigen p45 |
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00143-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: T. Pallidum recombinant antigen p45 (full length) |
T, Pallidum recombinant antigen p45 |
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00143-V-1000ug | Virogen | 1000 ug | EUR 1282.5 |
Description: T. Pallidum recombinant antigen p45 (full length) |
T, pallidum recombinant antigen TmpA |
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00144-V-01mg | Virogen | 0,1 mg | EUR 267.5 |
Description: T. Pallidum recombinant antigen TmpA (full length) |
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