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Category: vector

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

Posted on June 29, 2021April 29, 2021 by Leah

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript… Continue reading

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