Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control.
The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.
Pig Adrenal, Whole Total RNA* |
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| PR-501 | Zyagen | 0.05mg | EUR 235 |
Rat Adrenal Whole tissue lysate |
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| RAL-1470 | Alpha Diagnostics | 100ug | EUR 255.6 |
Pig Adrenal, Whole Total Protein |
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| PT-501 | Zyagen | 0.5mg | EUR 153 |
Mouse Adrenal Whole tissue lysate |
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| MAL-1417 | Alpha Diagnostics | 100ug | EUR 255.6 |
Bovine Eye, Whole cDNA |
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| BD-106 | Zyagen | 30 reactions | EUR 243 |
Human Normal Adrenal Gland Whole tissue lysate |
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| HALX-11050 | Alpha Diagnostics | 1 mg | EUR 927.6 |
Monkey (Cyno.) Normal Adrenal Gland Whole tissue lysate |
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| MCL-1160 | Alpha Diagnostics | 1 mg | EUR 927.6 |
PC-12 (Rat adrenal pheochromocytoma) Whole Cell Lysate |
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| E45O00018 | EnoGene | each | EUR 395 |
Monkey (Rhesus) Normal Adrenal Gland Whole tissue lysate |
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| MRL-1267 | Alpha Diagnostics | 1 mg | EUR 927.6 |
Adrenal Gland, Bovine |
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| MBS639367-1Each | MyBiosource | 1Each | EUR 510 |
Adrenal Gland, Bovine |
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| MBS639367-5x1Each | MyBiosource | 5x1Each | EUR 2080 |
Dog Adrenal cDNA* |
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| DD-501 | Zyagen | 30 Reactions | EUR 319 |
Cat Adrenal cDNA |
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| FD-501 | Zyagen | 30 Reactions | EUR 319 |
Rat Adrenal cDNA * |
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| RD-501 | Zyagen | 30 reactions | EUR 280 |
Sheep Adrenal cDNA* |
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| SD-501 | Zyagen | 30 reactions | EUR 280 |
57101-2 BOVINE ADRENAL 50 EA* |
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| 57101-2 | Pel-Freez | 50 Each | EUR 917 |
Bovine Adrenal, Cortex Total RNA* |
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| BR-506 | Zyagen | 0.05mg | EUR 235 |
Rabbit Adrenal cDNA |
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| TD-501 | Zyagen | 30 reactions | EUR 243 |
Bovine Adrenal, Medulla Total RNA* |
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| BR-507 | Zyagen | 0.05mg | EUR 235 |
Hamster Adrenal cDNA |
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| AD-501 | Zyagen | 30 reactions | EUR 243 |
MiniPig Adrenal cDNA |
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| ND-501 | Zyagen | 30 reactions | EUR 407 |
Bovine Adrenal, Cortex Total Protein |
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| BT-506 | Zyagen | 0.5mg | EUR 153 |
Bovine Adrenal, Medulla Total Protein |
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| BT-507 | Zyagen | 0.5mg | EUR 153 |
Bovine Adrenal Medulla 22P (BAM-22P) |
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| MBS633930-1mg | MyBiosource | 1mg | EUR 610 |
Bovine Adrenal Medulla 22P (BAM-22P) |
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| MBS633930-5x1mg | MyBiosource | 5x1mg | EUR 2600 |
Bovine Adrenal Medulla 18P (BAM-18P) |
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| MBS658691-1mg | MyBiosource | 1mg | EUR 610 |
Bovine Adrenal Medulla 18P (BAM-18P) |
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| MBS658691-5x1mg | MyBiosource | 5x1mg | EUR 2600 |
Bovine Adrenal Medulla 12P (BAM-12P) |
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| MBS659233-1mg | MyBiosource | 1mg | EUR 610 |
Bovine Adrenal Medulla 12P (BAM-12P) |
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| MBS659233-5x1mg | MyBiosource | 5x1mg | EUR 2600 |
Mouse CD1 Adrenal cDNA * |
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| MD-501 | Zyagen | 30 reactions | EUR 280 |
Mouse BLC Adrenal cDNA * |
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| MD-501-BLC | Zyagen | 30 reactions | EUR 319 |
Mouse C57 Adrenal cDNA * |
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| MD-501-C57 | Zyagen | 30 reactions | EUR 319 |
Guinea Pig Adrenal cDNA |
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| GD-501 | Zyagen | 30 reactions | EUR 243 |
Bovine Adrenal Microvascular Endothelial Cells |
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| B1013 | Alphabioregen | 1ml frozen Vial | EUR 0.2 |
Bovine Adrenal Microvascular Endothelial Cells |
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| cAP-b0008 | Angio Proteomie | 1Frozen Vial | EUR 742.5 |
Monkey Adrenal cDNA, Rhesus |
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| UD-501 | Zyagen | 30 reactions | EUR 446 |
BAM-12P, Bovine Adrenal Medulla Docosapeptide |
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| 5-00748 | CHI Scientific | 4 x 1mg | EUR 120.12 |
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Description: Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu |
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Monkey Adrenal cDNA, Cynomolgus |
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| KD-501 | Zyagen | 30 reactions | EUR 446 |
Bovine adrenal cortex antibody, ACA ELISA Kit |
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| MBS7239599-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Bovine adrenal cortex antibody, ACA ELISA Kit |
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| MBS7239599-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Bovine adrenal cortex antibody, ACA ELISA Kit |
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| MBS7239599-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Bovine adrenal cortex antibody, ACA ELISA Kit |
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| MBS7239599-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
Bovine adrenal cortex antibody,ACA ELISA kit |
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| E01A79939 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Bovine adrenal cortex antibody,ACA ELISA kit |
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| E11A2028-48wellsplate | BlueGene | 48 wells plate | EUR 280 |
Bovine adrenal cortex antibody,ACA ELISA kit |
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| E11A2028-96wellsplate | BlueGene | 96 wells plate | EUR 405 |
Bovine Adrenal Medulla 12P (BAM-12P) [7-12] |
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| MBS658494-1mg | MyBiosource | 1mg | EUR 610 |
Bovine Adrenal Medulla 12P (BAM-12P) [7-12] |
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| MBS658494-5x1mg | MyBiosource | 5x1mg | EUR 2600 |
cDNA - Human Tumor Tissue: Adrenal |
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| C1235004-10 | Biochain | 10 reactions | EUR 221.2 |
Human Adrenal cDNA-Oligo-dT |
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| HD-501 | Zyagen | 30 reactions | EUR 280 |
Rat Adrenal cDNA-Random Primer * |
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| RD-501-RH | Zyagen | 30 reactions | EUR 280 |
Human Adrenal cDNA-Random Primer |
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| HD-501-HR | Zyagen | 30 reactions | EUR 280 |
Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARFX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARFX2 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARPX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARPX2 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARSX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARSX2 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARZX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGBOGLARZX2 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARFX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARFX2 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARPX1 | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARPX2 | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARSX1 | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARSX2 | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARZX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Innovative Grade US Origin Bovine Gland Adrenal |
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| IGCLFGLARZX2 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal |
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Rat WS Adrenal cDNA-Oligo-dT * |
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| RD-501-WS | Zyagen | 30 reactions | EUR 280 |
cDNA - Human Adult Normal Tissue: Adrenal |
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| C1234004 | Biochain | 40 reactions | EUR 311.5 |
Mouse C57 Adrenal cDNA-Random Primer * |
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| MD-501-C57-RH | Zyagen | 30 reactions | EUR 319 |
Mouse CD1 Adrenal cDNA-Random Primer * |
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| MD-501-HR | Zyagen | 30 reactions | EUR 280 |
Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGBOGLARCFX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGBOGLARCPX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGBOGLARCSX1 | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGBOGLARCZX1 | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGCLFGLARCFX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGCLFGLARCPX1 | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGCLFGLARCSX1 | Innovative research | each | EUR 210 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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| IGCLFGLARCZX1 | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Gland Adrenal Cortex |
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cDNA - Monkey (Rhesus) Normal Tissue: Adrenal |
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| C1534004 | Biochain | 40 reactions | EUR 531.3 |
Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
| IGBOGLARMFX1 | Innovative research | each | EUR 210 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
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Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
| IGBOGLARMPX1 | Innovative research | each | EUR 210 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
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Innovative Grade US Origin Bovine Gland Adrenal Medulla |
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| IGBOGLARMSX1 | Innovative research | each | EUR 133 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
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Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
| IGBOGLARMZX1 | Innovative research | each | EUR 133 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
| IGCLFGLARMFX1 | Innovative research | each | EUR 133 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
| IGCLFGLARMPX1 | Innovative research | each | EUR 210 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
| IGCLFGLARMSX1 | Innovative research | each | EUR 210 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
Innovative Grade US Origin Bovine Gland Adrenal Medulla |
|||
| IGCLFGLARMZX1 | Innovative research | each | EUR 133 |
|
Description: Innovative Grade US Origin Bovine Gland Adrenal Medulla |
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Monkey Adrenal cDNA-Random Primer, Rhesus |
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| UD-501-RH | Zyagen | 30 reactions | EUR 446 |
cDNA - Monkey (Cynomolgus) Normal Tissue: Adrenal |
|||
| C1534004-Cy | Biochain | 40 reactions | EUR 531.3 |
Monkey Adrenal cDNA-Random Primer, Cynomolgus |
|||
| KD-501-RH | Zyagen | 30 reactions | EUR 446 |
Peptide E / 3200-Dalton Adrenal Enkephalin-Containing Peptide (Bovine) |
|||
| 024-57 | PHOENIX PEPTIDE | 200 μg | EUR 86.4 |
Tissue cDNA, First Strand, Human Tumor, Adrenal, BioGenomics |
|||
| MBS652063-10Tests | MyBiosource | 10Tests | EUR 570 |
Tissue cDNA, First Strand, Human Tumor, Adrenal, BioGenomics |
|||
| MBS652063-5x10Tests | MyBiosource | 5x10Tests | EUR 2345 |
BOVINE, EYE, WHOLE, FRESH |
|||
| 8600822 | Lampire Biologicals Laboratories | 1EA | EUR 30.49 |
BOVINE, EYE, WHOLE, FROZEN |
|||
| 8620822 | Lampire Biologicals Laboratories | 1EA | EUR 30.49 |
Tissue cDNA, First Strand, Human Adult Normal, Adrenal, BioGenomics |
|||
| MBS651970-40Tests | MyBiosource | 40Tests | EUR 680 |
Tissue cDNA, First Strand, Human Adult Normal, Adrenal, BioGenomics |
|||
| MBS651970-5x40Tests | MyBiosource | 5x40Tests | EUR 2835 |
Pig Eye, Whole cDNA |
|||
| PD-106 | Zyagen | 30 reactions | EUR 243 |
Sheep Eye, whole cDNA |
|||
| SD-106 | Zyagen | 30 reactions | EUR 243 |
Rat Heart, whole cDNA |
|||
| RD-801 | Zyagen | 30 reactions | EUR 243 |
Bovine Eye, Whole Total RNA |
|||
| BR-106 | Zyagen | 0.1mg | EUR 160 |
MiniPig Eye, Whole cDNA |
|||
| ND-106 | Zyagen | 30 reactions | EUR 358 |
Rat Stomach, whole cDNA |
|||
| RD-302 | Zyagen | 30 reactions | EUR 243 |
Equine Brain, whole cDNA |
|||
| ED-201 | Zyagen | 30 reactions | EUR 319 |
Rabbit Brain, whole cDNA |
|||
| TD-201 | Zyagen | 30 reactions | EUR 243 |
Chicken Brain, Whole cDNA |
|||
| CD-201 | Zyagen | 30 reactions | EUR 243 |
Rat Intestine, whole cDNA |
|||
| RD-306 | Zyagen | 30 reactions | EUR 243 |
Rat Epididymis, whole cDNA |
|||
| RD-402 | Zyagen | 30 reactions | EUR 243 |
Bovine Eye, Whole Total Protein |
|||
| BT-106 | Zyagen | 1mg | EUR 153 |
Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Adrenal, BioGenomics |
|||
| MBS652504-40Tests | MyBiosource | 40Tests | EUR 850 |
Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Adrenal, BioGenomics |
|||
| MBS652504-5x40Tests | MyBiosource | 5x40Tests | EUR 3600 |
Immunoglobulin M (IgM) Whole Bovine |
|||
| GWB-97A00C | GenWay Biotech | 1 mg | Ask for price |
Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Adrenal, BioGenomics |
|||
| MBS652357-40Tests | MyBiosource | 40Tests | EUR 850 |
Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Adrenal, BioGenomics |
|||
| MBS652357-5x40Tests | MyBiosource | 5x40Tests | EUR 3600 |
Bovine Adrenaline ELISA Kit |
|||
| QY-E60146 | Qayee Biotechnology | 96T | EUR 511.2 |
Mouse CD1 Brain, whole cDNA |
|||
| MD-201 | Zyagen | 30 reactions | EUR 243 |
Mouse C57 Brain, whole cDNA |
|||
| MD-201-C57-1 | Zyagen | 30 reactions | EUR 280 |
Mouse CD1 Heart, whole cDNA |
|||
| MD-801 | Zyagen | 30 reactions | EUR 243 |
Mouse C57 Heart, whole cDNA |
|||
| MD-801-C57 | Zyagen | 30 reactions | EUR 280 |
Rat Spinal cord, whole cDNA |
|||
| RD-230 | Zyagen | 30 reactions | EUR 243 |
Adrenal gland disease spectrum (adrenal gland cancer progression) tissue array (2017 WHO classification) |
|||
| AD2081a | TissueArray | each | EUR 546 |
|
Description: Adrenal gland disease spectrum (adrenal gland cancer progression) tissue array (2017 WHO classification), including TNM and clinical stage (AJCC 7th edition), 96 cases/192 cores, replacing AD2081 |
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Guinea Pig Brain, whole cDNA |
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| GD-201 | Zyagen | 30 reactions | EUR 243 |
Mouse CD1 Stomach, whole cDNA |
|||
| MD-302 | Zyagen | 30 reactions | EUR 243 |
Mouse BLC Stomach, whole cDNA |
|||
| MD-302-BLC | Zyagen | 30 reactions | EUR 280 |
Mouse C57 Stomach, whole cDNA |
|||
| MD-302-C57 | Zyagen | 30 reactions | EUR 280 |
Mouse CD1 Intestine, whole cDNA |
|||
| MD-306 | Zyagen | 30 reactions | EUR 243 |
Mouse BLC Intestine, whole cDNA |
|||
| MD-306-BLC | Zyagen | 30 reactions | EUR 280 |
Mouse C57 Intestine, whole cDNA |
|||
| MD-306-C57 | Zyagen | 30 reactions | EUR 280 |
Mouse CD1 Epididymis, whole cDNA |
|||
| MD-402 | Zyagen | 30 reactions | EUR 243 |
Mouse BLC Epididymis, whole cDNA |
|||
| MD-402-BLC | Zyagen | 30 reactions | EUR 280 |
Mouse C57 Epididymis, whole cDNA |
|||
| MD-402-C57 | Zyagen | 30 reactions | EUR 280 |
Adrenal tumors with normal adrenal tissue and matched cancer adjacent adrenal tissue array |
|||
| AG991 | TissueArray | each | EUR 356 |
|
Description: Adrenal tumors with normal adrenal tissue and matched cancer adjacent adrenal tissue array, including TNM and clinical stage (AJCC 8th edition), 93 cases/99 cores (core size 1.5mm) |
|||
Adrenal Dissociation System 9 (Adrenal), Mouse and Rat |
|||
| 4-20209 | CHI Scientific | ea | EUR 195.92 |
Mouse CD1 Spinal cord, whole cDNA |
|||
| MD-230 | Zyagen | 30 reactions | EUR 243 |
Mouse BLC Spinal cord, whole cDNA |
|||
| MD-230-BLC | Zyagen | 30 reactions | EUR 280 |
Mouse C57 Spinal cord, whole cDNA |
|||
| MD-230-C57 | Zyagen | 30 reactions | EUR 280 |
Bovine IgG Whole Molecule-Biotin Conjugate |
|||
| MBS689526-1mg | MyBiosource | 1mg | EUR 185 |
Bovine IgG Whole Molecule-Biotin Conjugate |
|||
| MBS689526-5x1mg | MyBiosource | 5x1mg | EUR 670 |
Adrenal Lysate |
|||
| 1417 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Adrenal Lysate |
|||
| 1470 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Adrenal Lysate |
|||
| 21-160 | ProSci | 0.1 mg | EUR 468.6 |
|
Description: Monkey (Cynomolgus) adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) adrenal tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Adrenal Lysate |
|||
| MBS151591-01mg | MyBiosource | 0.1mg | EUR 200 |
Adrenal Lysate |
|||
| MBS151591-5x01mg | MyBiosource | 5x0.1mg | EUR 880 |
Adrenal Lysate |
|||
| MBS151750-01mg | MyBiosource | 0.1mg | EUR 200 |
Adrenal Lysate |
|||
| MBS151750-5x01mg | MyBiosource | 5x0.1mg | EUR 880 |
Adrenal Lysate |
|||
| XBL-11050 | ProSci | 0.1 mg | EUR 663.9 |
|
Description: Human adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human adrenal tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Rat Brain, whole Tissue cDNA |
|||
| RD-201 | Zyagen | 30 reactions | EUR 243 |
Rat Heart, whole cDNA-Random Primer |
|||
| RD-801-RH | Zyagen | 30 reactions | EUR 243 |
Rat Brain, whole cDNA-Random Primer |
|||
| RD-201-RH | Zyagen | 30 reactions | EUR 243 |
Rat Stomach, whole cDNA-Random Primer |
|||
| RD-302-RH | Zyagen | 30 reactions | EUR 243 |
Innovative Grade US Origin Bovine Whole Blood |
|||
| IGBOWBK2E1000ML | Innovative research | each | EUR 312 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
|||
Innovative Grade US Origin Bovine Whole Blood |
|||
| IGBOWBK2E100ML | Innovative research | each | EUR 171 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
|||
Innovative Grade US Origin Bovine Whole Blood |
|||
| IGBOWBK2E500ML | Innovative research | each | EUR 230 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
|||
Innovative Grade US Origin Bovine Whole Blood |
|||
| IGBOWBK2E50ML | Innovative research | each | EUR 136 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
|||
Innovative Grade US Origin Bovine Whole Blood |
|||
| IGBOWBK3E1000ML | Innovative research | each | EUR 312 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBK3E100ML | Innovative research | each | EUR 171 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBK3E500ML | Innovative research | each | EUR 230 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBK3E50ML | Innovative research | each | EUR 136 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOF1000ML | Innovative research | each | EUR 312 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOF100ML | Innovative research | each | EUR 171 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOF500ML | Innovative research | each | EUR 230 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOF50ML | Innovative research | each | EUR 136 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOX1000ML | Innovative research | each | EUR 312 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOX100ML | Innovative research | each | EUR 171 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOX500ML | Innovative research | each | EUR 230 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBKOX50ML | Innovative research | each | EUR 136 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBLIH1000ML | Innovative research | each | EUR 312 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBLIH100ML | Innovative research | each | EUR 171 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBLIH500ML | Innovative research | each | EUR 230 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBLIH50ML | Innovative research | each | EUR 136 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBNAC1000ML | Innovative research | each | EUR 312 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBNAC100ML | Innovative research | each | EUR 171 |
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Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBNAC500ML | Innovative research | each | EUR 230 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBNAC50ML | Innovative research | each | EUR 136 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBNAE1000ML | Innovative research | each | EUR 312 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
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Innovative Grade US Origin Bovine Whole Blood |
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| IGBOWBNAE100ML | Innovative research | each | EUR 171 |
|
Description: Innovative Grade US Origin Bovine Whole Blood |
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A new mathematical model for relative quantification in real-time RT-PCR.
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as well as rapid quantification results. But accurate quantification of nucleic acids requires a reproducible methodology and an adequate mathematical model for data analysis.
This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript. Therefore, a new mathematical model is presented. The relative expression ratio is calculated only from the real-time PCR efficiencies and the crossing point deviation of an unknown sample versus a control. This model needs no calibration curve. Control levels were included in the model to standardise each reaction run with respect to RNA integrity, sample loading and inter-PCR variations. High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model.
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage lambda Red recombinase, which is synthesized under the control of an inducible promoter on an easily curable, low copy number plasmid. To demonstrate the utility of this approach, we generated PCR products by using primers with 36- to 50-nt extensions that are homologous to regions adjacent to the gene to be inactivated and template plasmids carrying antibiotic resistance genes that are flanked by FRT (FLP recognition target) sites. By using the respective PCR products, we made 13 different disruptions of chromosomal genes.
Mutants of the arcB, cyaA, lacZYA, ompR-envZ, phnR, pstB, pstCA, pstS, pstSCAB-phoU, recA, and torSTRCAD genes or operons were isolated as antibiotic-resistant colonies after the introduction into bacteria carrying a Red expression plasmid of synthetic (PCR-generated) DNA. The resistance genes were then eliminated by using a helper plasmid encoding the FLP recombinase which is also easily curable. This procedure should be widely useful, especially in genome analysis of E. coli and other bacteria because the procedure can be done in wild-type cells.
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
BACKGROUND
Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large dynamic range of this methodology, the requirements for a proper internal control gene for normalization have become increasingly stringent. Although housekeeping gene expression has been reported to vary considerably, no systematic survey has properly determined the errors related to the common practice of using only one control gene, nor presented an adequate way of working around this problem.
RESULTS
We outline a robust and innovative strategy to identify the most stably expressed control genes in a given set of tissues, and to determine the minimum number of genes required to calculate a reliable normalization factor. We have evaluated ten housekeeping genes from different abundance and functional classes in various human tissues, and demonstrated that the conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested. The geometric mean of multiple carefully selected housekeeping genes was validated as an accurate normalization factor by analyzing publicly available microarray data.
Analyzing real-time PCR data by the comparative C(T) method.
Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene.
A widely used method to present relative gene expression is the comparative C(T) method also referred to as the 2 (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative C(T) method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.
anti-PD-L1 VHH antibody (Alexa Fluor® 488) |
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| JOT0002-5-3-50 | Jotbody | 50 | EUR 340.96 |
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Description: Conjugated VHH single domain antibody |
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anti-PD-L1 VHH antibody (Alexa Fluor® 488) |
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| SB-JOT0002-5-3-100 | Jotbody | 100 (25 x 4) | EUR 340.9 |
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Description: Conjugated VHH single domain antibody |
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anti-PD-L1 VHH antibody (Alexa Fluor® 488) |
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| SB-JOT0002-5-3-5-25 | Jotbody | 25 | EUR 170.8 |
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Description: Conjugated VHH single domain antibody |
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SAM FCM (Alexa Fluor 488) |
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| abx098904-60tests | Abbexa | 60 tests | EUR 1629.6 |
Anti-EGFR Alexa Fluor® 488 |
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| A4-680-T100 | ExBio | 100tests | EUR 242 |
CD40 (human) monoclonal antibody (mAb 89) (Alexa Fluor 488) |
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| MBS566768-01mg | MyBiosource | 0.1mg | EUR 620 |
CD40 (human) monoclonal antibody (mAb 89) (Alexa Fluor 488) |
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| MBS566768-5x01mg | MyBiosource | 5x0.1mg | EUR 2740 |
Anti-Hu CD16 Alexa Fluor 488 |
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| MBS1801989-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Alexa Fluor 488 anti-human CD3 |
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| MBS9459431-25Tests | MyBiosource | 25Tests | EUR 230 |
Alexa Fluor 488 anti-human CD3 |
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| MBS9459431-50Tests | MyBiosource | 50Tests | EUR 290 |
Alexa Fluor 488 anti-human CD3 |
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| MBS9459431-5x50Tests | MyBiosource | 5x50Tests | EUR 1160 |
Alexa Fluor 488 anti-human CD3 |
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| MBS9459432-25Tests | MyBiosource | 25Tests | EUR 230 |
Alexa Fluor 488 anti-human CD3 |
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| MBS9459432-50Tests | MyBiosource | 50Tests | EUR 290 |
Alexa Fluor 488 anti-human CD3 |
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| MBS9459432-5x50Tests | MyBiosource | 5x50Tests | EUR 1160 |
Alexa Fluor® 488 Streptavidin |
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| E16FXP040G | EnoGene | 100 μg | EUR 758.33 |
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Description: Available in various conjugation types. |
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Anti-Rat CD8a Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558639-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Rat CD8a Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558639-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Rat CD8a Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558639-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Rat CD8a Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558639-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559102-100Tests | MyBiosource | 100Tests | EUR 235 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559102-200Tests | MyBiosource | 200Tests | EUR 300 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559102-20Tests | MyBiosource | 20Tests | EUR 140 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559102-5x200Tests | MyBiosource | 5x200Tests | EUR 1350 |
Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553692-100Tests | MyBiosource | 100Tests | EUR 130 |
Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553692-200Tests | MyBiosource | 200Tests | EUR 155 |
Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553692-50Tests | MyBiosource | 50Tests | EUR 110 |
Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553692-5x200Tests | MyBiosource | 5x200Tests | EUR 685 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553734-100Tests | MyBiosource | 100Tests | EUR 165 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553734-200Tests | MyBiosource | 200Tests | EUR 215 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553734-20Tests | MyBiosource | 20Tests | EUR 115 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553734-5x200Tests | MyBiosource | 5x200Tests | EUR 950 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553774-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553774-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553774-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553774-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553912-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553912-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553912-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553912-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553956-100Tests | MyBiosource | 100Tests | EUR 165 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553956-200Tests | MyBiosource | 200Tests | EUR 215 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553956-20Tests | MyBiosource | 20Tests | EUR 115 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2553956-5x200Tests | MyBiosource | 5x200Tests | EUR 950 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556456-100Tests | MyBiosource | 100Tests | EUR 250 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556456-200Tests | MyBiosource | 200Tests | EUR 330 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556456-20Tests | MyBiosource | 20Tests | EUR 150 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556456-5x200Tests | MyBiosource | 5x200Tests | EUR 1470 |
Anti-Mouse Ly6C Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556940-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse Ly6C Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556940-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse Ly6C Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556940-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse Ly6C Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556940-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse CD45.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556947-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD45.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556947-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD45.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556947-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD45.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2556947-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2557986-100Tests | MyBiosource | 100Tests | EUR 185 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2557986-200Tests | MyBiosource | 200Tests | EUR 235 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2557986-50Tests | MyBiosource | 50Tests | EUR 140 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2557986-5x200Tests | MyBiosource | 5x200Tests | EUR 1045 |
Anti-Mouse CD22 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558155-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD22 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558155-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD22 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558155-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD22 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558155-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558203-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558203-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558203-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558203-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Human CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558291-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558291-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558291-20Tests | MyBiosource | 20Tests | EUR 140 |
Anti-Human CD40 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558291-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Human CD35 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558413-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD35 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558413-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD35 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558413-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD35 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558413-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Human CD37 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558422-100Tests | MyBiosource | 100Tests | EUR 265 |
Anti-Human CD37 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558422-200Tests | MyBiosource | 200Tests | EUR 360 |
Anti-Human CD37 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558422-20Tests | MyBiosource | 20Tests | EUR 155 |
Anti-Human CD37 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558422-5x200Tests | MyBiosource | 5x200Tests | EUR 1610 |
Anti-Human CD74 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558449-100Tests | MyBiosource | 100Tests | EUR 265 |
Anti-Human CD74 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558449-200Tests | MyBiosource | 200Tests | EUR 360 |
Anti-Human CD74 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558449-20Tests | MyBiosource | 20Tests | EUR 155 |
Anti-Human CD74 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558449-5x200Tests | MyBiosource | 5x200Tests | EUR 1610 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558588-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558588-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558588-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558588-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558679-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558679-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558679-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558679-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Mouse CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2558983-100Tests | MyBiosource | 100Tests | EUR 135 |
Anti-Mouse CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2558983-200Tests | MyBiosource | 200Tests | EUR 180 |
Anti-Mouse CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2558983-50Tests | MyBiosource | 50Tests | EUR 115 |
Anti-Mouse CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2558983-5x200Tests | MyBiosource | 5x200Tests | EUR 795 |
Anti-Human CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559003-100Tests | MyBiosource | 100Tests | EUR 165 |
Anti-Human CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559003-200Tests | MyBiosource | 200Tests | EUR 215 |
Anti-Human CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559003-20Tests | MyBiosource | 20Tests | EUR 130 |
Anti-Human CD45 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559003-5x200Tests | MyBiosource | 5x200Tests | EUR 950 |
Anti-Human CD27 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559034-100Tests | MyBiosource | 100Tests | EUR 165 |
Anti-Human CD27 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559034-200Tests | MyBiosource | 200Tests | EUR 215 |
Anti-Human CD27 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559034-20Tests | MyBiosource | 20Tests | EUR 115 |
Anti-Human CD27 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559034-5x200Tests | MyBiosource | 5x200Tests | EUR 950 |
Anti-Human CD10 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559041-100Tests | MyBiosource | 100Tests | EUR 265 |
Anti-Human CD10 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559041-200Tests | MyBiosource | 200Tests | EUR 360 |
Anti-Human CD10 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559041-20Tests | MyBiosource | 20Tests | EUR 160 |
Anti-Human CD10 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559041-5x200Tests | MyBiosource | 5x200Tests | EUR 1610 |
Anti-Human CD15 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559051-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD15 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559051-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD15 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559051-20Tests | MyBiosource | 20Tests | EUR 140 |
Anti-Human CD15 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559051-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Mouse CD31 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
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| MBS2559281-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Mouse CD31 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559281-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Mouse CD31 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559281-50Tests | MyBiosource | 50Tests | EUR 165 |
Anti-Mouse CD31 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
|||
| MBS2559281-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |