Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control.
The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.
cDNA from Monkey (Rhesus) Normal Tissue: Adrenal |
|||
| C1534004 | Biochain | 40 reactions | EUR 540 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal |
|||
| C1534004-Cy | Biochain | 40 reactions | EUR 540 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
Monkey (Cyno.) Normal Adrenal Gland Whole tissue lysate |
|||
| MCL-1160 | Alpha Diagnostics | 1 mg | EUR 773 |
Monkey (Rhesus) Normal Adrenal Gland Whole tissue lysate |
|||
| MRL-1267 | Alpha Diagnostics | 1 mg | EUR 773 |
cDNA from Human Diabetic Tissue: Whole Eye |
|||
| C1236108Dia-10 | Biochain | 10 reactions | EUR 383 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Human Adult Normal Tissue: Whole Eye |
|||
| C1234108-10 | Biochain | 10 reactions | EUR 446 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Monkey (Cynomolgus) Normal Tissue: Whole Eye |
|||
| C1534108-Cy | Biochain | 40 reactions | EUR 540 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
BAM-12P, Bovine Adrenal Medulla Docosapeptide |
|||
| 5-00748 | CHI Scientific | 4 x 1mg | Ask for price |
Bovine EYE WHOLE 25 EA* |
|||
| 57111-2 | Pel-Freez | 25 EA | EUR 295.55 |
Bovine KIDNEY WHOLE 2 EA* |
|||
| 57120-2 | Pel-Freez | 2 EA | EUR 174.84 |
Bovine PITUITARY WHOLE 25 EA* |
|||
| 57133-2 | Pel-Freez | 25 EA | EUR 597.88 |
Adrenal Lysate |
|||
| 1417 | ProSci | 0.1 mg | EUR 191 |
|
Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Adrenal Lysate |
|||
| 1470 | ProSci | 0.1 mg | EUR 191 |
|
Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Adrenal Lysate |
|||
| 21-160 | ProSci | 0.1 mg | EUR 390.5 |
|
Description: Monkey (Cynomolgus) adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) adrenal tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
|||
Adrenal Lysate |
|||
| XBL-11050 | ProSci | 0.1 mg | EUR 553.25 |
|
Description: Human adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human adrenal tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
|||
Adrenal Dissociation System 9 (Adrenal), Mouse and Rat |
|||
| 4-20209 | CHI Scientific | ea | Ask for price |
Paraffin Tissue Section - Human Adrenal Tumor: Adrenal Pheochromocytoma |
|||
| T2235004-2 | Biochain | 5 slides | EUR 257 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
Adrenal Lupus Lysate |
|||
| XBL-10316 | ProSci | 0.1 mg | EUR 663.5 |
|
Description: Human adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human adrenal tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
|||
Adrenal Cytoplasmic Lysate |
|||
| XBL-10453 | ProSci | 0.1 mg | EUR 227.75 |
|
Description: Human adrenal tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human adrenal tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated adrenal tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated adrenal tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
|||
Adrenal Membrane Lysate |
|||
| XBL-10454 | ProSci | 0.1 mg | EUR 737 |
|
Description: Human adrenal tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human adrenal tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated adrenal tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated adrenal tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
|||
Rabbit anti Whole Bovine serum antibody (IgG fraction) |
|||
| 40R-RB002 | Fitzgerald | 20 mg | EUR 327 |
|
Description: Affinity purified Rabbit polyclonal Rabbit anti Whole Bovine serum antibody (IgG fraction) |
|||
Adrenal Dissociation System 2 (Leydig, Adrenal), Mouse and Rat |
|||
| 4-20202 | CHI Scientific | ea | Ask for price |
Rabbit Anti-Bovine IgG (whole IgG from colostrum), unlabeled |
|||
| 80516 | Alpha Diagnostics | 1 ml | EUR 202 |
Rabbit Anti-Bovine IgG (whole IgG from serum), unlabeled |
|||
| 80517 | Alpha Diagnostics | 1 ml | EUR 202 |
Pig ADRENAL 50 EA* |
|||
| 59401-2 | Pel-Freez | 50 EA | EUR 440.19 |
Rabbit ADRENAL 50 EA |
|||
| 41201-2 | Pel-Freez | 50 EA | EUR 341.23 |
Adrenal Liver Cirrhosis Lysate |
|||
| XBL-10315 | ProSci | 0.1 mg | EUR 663.5 |
|
Description: Human adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human adrenal tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
|||
Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat |
|||
| 4-20204 | CHI Scientific | ea | Ask for price |
Histone (Whole) Antibody |
|||
| abx023918-2ml | Abbexa | 2 ml | EUR 509 |
Rat Whole Heart |
|||
| PC35133 | Neuromics | P0 Rat - Whole Heart X2 | EUR 1341 |
Mouse Whole Heart |
|||
| PC35135 | Neuromics | P0 Mouse - Whole heart X2 | EUR 1341 |
Rabbit ADRENAL MATURE 50 EA |
|||
| 41301-2 | Pel-Freez | 50 EA | EUR 341.23 |
Total Protein from Lupus: Adrenal |
|||
| P1236004Lup | Biochain | 1 mg | EUR 461 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
Frozen Tissue Section - Lupus: Adrenal |
|||
| T1236004Lup | Biochain | 5 slides | EUR 474 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
cDNA Synthesis SuperMix |
|||
| 20-abx09801420ulSystems | Abbexa |
|
|
Novo? cDNA Kit |
|||
| M1165-100 | Biovision | EUR 354 | |
Novo? cDNA Kit |
|||
| M1165-25 | Biovision | EUR 267 | |
Evo? cDNA Supermix |
|||
| M1168-100 | Biovision | EUR 381 | |
Evo? cDNA Supermix |
|||
| M1168-25 | Biovision | EUR 267 | |
Novo? cDNA Supermix |
|||
| M1169-100 | Biovision | EUR 441 | |
Novo? cDNA Supermix |
|||
| M1169-25 | Biovision | EUR 289 | |
Whole Blood PCR Mix |
|||
| M1143-200 | Biovision | EUR 321 | |
Whole Human Serum antibody |
|||
| 20-1727 | Fitzgerald | 1 L | EUR 30 |
|
Description: Goat polyclonal Whole Human Serum antibody |
|||
Whole Human Serum antibody |
|||
| 20-S1110G000-V0 | Fitzgerald | 10 ml | EUR 133 |
|
Description: Goat polyclonal Whole Human Serum antibody |
|||
CMV protein (whole cell) |
|||
| 30-AC70 | Fitzgerald | 1 ml | EUR 403 |
|
Description: Purified native CMV protein (whole cell) |
|||
Monkey IgM (whole molecule) |
|||
| 31R-AI093 | Fitzgerald | 250 µg | EUR 417 |
|
Description: Monkey whole IgM |
|||
Human adrenal cortex antibody ELISA Kit |
|||
| ELA-E0342h | Lifescience Market | 96 Tests | EUR 824 |
Total Protein from Liver Cirrhosis: Adrenal |
|||
| P1236004Lcs | Biochain | 1 mg | EUR 461 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
Frozen Tissue Section - Human Tumor: Adrenal |
|||
| T1235004 | Biochain | 5 slides | EUR 338 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
Paraffin Tissue Section - Human Adrenal Tumor |
|||
| T2235004 | Biochain | 5 slides | EUR 257 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
|||
| C1634310 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
|||
| C1634330 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
|||
| C1634340 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
|||
| C1634350 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
|||
| C1634360 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
|||
| C1634390 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure |
|||
| HCG14-A | Alpha Diagnostics | 1 mg | EUR 286 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
|||
| 20-abx09801620ulSystems | Abbexa |
|
|
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
|||
| 20-abx09802120ulSystems | Abbexa |
|
|
cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
|||
| C1634370 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA Probe Diluent Solution |
|||
| AR0063 | BosterBio | 5mL | EUR 106 |
Tetro cDNA Synthesis Kit |
|||
| BIO-65042 | Bioline | 30 Reactions | Ask for price |
Tetro cDNA Synthesis Kit |
|||
| BIO-65043 | Bioline | 100 Reactions | Ask for price |
SensiFAST cDNA Synthesis Kit |
|||
| BIO-65053 | Bioline | 50 Reactions | Ask for price |
SensiFAST cDNA Synthesis Kit |
|||
| BIO-65053/S | Bioline | Sample | Ask for price |
SensiFAST cDNA Synthesis Kit |
|||
| BIO-65054 | Bioline | 250 Reactions | Ask for price |
Human eNOS cDNA probe |
|||
| eNOS51-D-2 | Alpha Diagnostics | 2 ug | EUR 445 |
Plant Tissue cDNA: Arabidopsis |
|||
| PC34-310 | Alpha Diagnostics | 10 rxn | EUR 415 |
OneScriptPlus cDNA Synthesis Kit |
|||
| G235 | ABM | 25 x 20 ul reactions | EUR 97 |
OneScriptPlus cDNA Synthesis Kit |
|||
| G236 | ABM | 100 x 20 ul reactions | EUR 169 |
OneScriptPlus cDNA Synthesis SuperMix |
|||
| G453 | ABM | 25 x 20 ul reactions | EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
|||
| G454 | ABM | 100 x 20 ul reactions | EUR 169 |
circRNA cDNA Synthesis Kit |
|||
| G627 | ABM | 25 rxn (20 ul/rxn) | EUR 309 |
Evo™ cDNA Kit |
|||
| M1164-100 | Biovision | Ask for price | |
Evo™ cDNA Kit |
|||
| M1164-25 | Biovision | Ask for price | |
Novo? Transcriptome cDNA Kit |
|||
| M1167-100 | Biovision | EUR 952 | |
Novo? Transcriptome cDNA Kit |
|||
| M1167-25 | Biovision | EUR 441 | |
cDNA from Arteriosclerosis: Aorta |
|||
| C1236012Hd-4 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from hypertension: Artery |
|||
| C1236013Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Arteriosclerosis: Artery |
|||
| C1236013Hd-4 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from hypertension: Vein |
|||
| C1236020Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Lupus: Colon |
|||
| C1236090Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from hypertension: Heart |
|||
| C1236122Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Lupus: Heart |
|||
| C1236122Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from hypertension: Kidney |
|||
| C1236142Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Lupus: Kidney |
|||
| C1236142Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Lupus: Liver |
|||
| C1236149Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Asthma: Lung |
|||
| C1236152Ld-1 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Bronchitis: Lung |
|||
| C1236152Ld-2 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Emphysema: Lung |
|||
| C1236152Ld-3 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Pneumonia: Lung |
|||
| C1236152Ld-4 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Lupus: Lung |
|||
| C1236152Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Lupus: Pancreas |
|||
| C1236188Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Lupus: Spleen |
|||
| C1236246Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
A new mathematical model for relative quantification in real-time RT-PCR.
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as well as rapid quantification results. But accurate quantification of nucleic acids requires a reproducible methodology and an adequate mathematical model for data analysis.
This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript. Therefore, a new mathematical model is presented. The relative expression ratio is calculated only from the real-time PCR efficiencies and the crossing point deviation of an unknown sample versus a control. This model needs no calibration curve. Control levels were included in the model to standardise each reaction run with respect to RNA integrity, sample loading and inter-PCR variations. High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model.
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage lambda Red recombinase, which is synthesized under the control of an inducible promoter on an easily curable, low copy number plasmid. To demonstrate the utility of this approach, we generated PCR products by using primers with 36- to 50-nt extensions that are homologous to regions adjacent to the gene to be inactivated and template plasmids carrying antibiotic resistance genes that are flanked by FRT (FLP recognition target) sites. By using the respective PCR products, we made 13 different disruptions of chromosomal genes.
Mutants of the arcB, cyaA, lacZYA, ompR-envZ, phnR, pstB, pstCA, pstS, pstSCAB-phoU, recA, and torSTRCAD genes or operons were isolated as antibiotic-resistant colonies after the introduction into bacteria carrying a Red expression plasmid of synthetic (PCR-generated) DNA. The resistance genes were then eliminated by using a helper plasmid encoding the FLP recombinase which is also easily curable. This procedure should be widely useful, especially in genome analysis of E. coli and other bacteria because the procedure can be done in wild-type cells.
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
BACKGROUND
Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large dynamic range of this methodology, the requirements for a proper internal control gene for normalization have become increasingly stringent. Although housekeeping gene expression has been reported to vary considerably, no systematic survey has properly determined the errors related to the common practice of using only one control gene, nor presented an adequate way of working around this problem.
RESULTS
We outline a robust and innovative strategy to identify the most stably expressed control genes in a given set of tissues, and to determine the minimum number of genes required to calculate a reliable normalization factor. We have evaluated ten housekeeping genes from different abundance and functional classes in various human tissues, and demonstrated that the conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested. The geometric mean of multiple carefully selected housekeeping genes was validated as an accurate normalization factor by analyzing publicly available microarray data.
Analyzing real-time PCR data by the comparative C(T) method.
Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene.
A widely used method to present relative gene expression is the comparative C(T) method also referred to as the 2 (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative C(T) method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.
AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
|||
| 16891 | AAT Bioquest | 1 mg | EUR 176 |
AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
|||
| 23153 | AAT Bioquest | 300 Tests | EUR 306 |
Anti-Hu CD72 Alexa Fluor® 488 |
|||
| A4-310-T100 | ExBio | 100 tests | EUR 269 |
Anti-Bov CD9 Alexa Fluor® 488 |
|||
| A4-354-C100 | ExBio | 0.1 mg | EUR 269 |
Endoglin/CD105 Alexa Fluor |
|||
| FC15024 | Neuromics | 100 Tests | EUR 448 |
Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
|||
| S0017 | Affbiotech | 200ul | EUR 304 |
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
|||
| S0018 | Affbiotech | 200ul | EUR 304 |
Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
|||
| M09406 | BosterBio | 100ug | EUR 565 |
|
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
|||
Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
|||
| Ab8032-001 | GenDepot | 0.5mg | EUR 435 |
Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
|||
| A4-686-C100 | ExBio | 0.1 mg | EUR 269 |
Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
|||
| A4-712-C100 | ExBio | 0.1 mg | EUR 269 |
Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
|||
| A4-730-C100 | ExBio | 0.1 mg | EUR 269 |
Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
|||
| A4-737-C100 | ExBio | 0.1 mg | EUR 269 |
Anti-LAMP3 antibody (Alexa-fluor 546) |
|||
| STJ170005 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
|||
Anti-LAMP3 antibody (Alexa-fluor 647) |
|||
| STJ170006 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
|||
Anti-IL3RA antibody (Alexa-fluor 546) |
|||
| STJ170010 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
|||
Anti-IL3RA antibody (Alexa-fluor 647) |
|||
| STJ170011 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
|||
Anti-CD207 antibody (Alexa-fluor 546) |
|||
| STJ170015 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
|||
Anti-CD207 antibody (Alexa-fluor 647) |
|||
| STJ170016 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
|||
Anti-IL7R antibody (Alexa-fluor 546) |
|||
| STJ170021 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R |
|||
Anti-IL7R antibody (Alexa-fluor 647) |
|||
| STJ170022 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R |
|||
SAM FCM (Alexa Fluor 647) |
|||
| abx098902-100tests | Abbexa | 100 tests | EUR 1233 |
Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488) |
|||
| M02316 | BosterBio | 100ug | EUR 580 |
|
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
|||
Alpha Fluor™ 488 amine |
|||
| 1705 | AAT Bioquest | 1 mg | EUR 306 |
Alpha Fluor™ 488 Hydroxylamine |
|||
| 1900 | AAT Bioquest | 1 mg | EUR 306 |
Alpha Fluor™ 488 NHS Ester |
|||
| 1812 | AAT Bioquest | 1 mg | EUR 219 |
Streptavidin-Alexa488 (Alexas fluor 488) conjugate |
|||
| SV-A488-100 | Alpha Diagnostics | 100 tests | EUR 225 |
Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
|||
| 50336 | Alpha Diagnostics | 0.5 ml | EUR 225 |
Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
|||
| 1795 | AAT Bioquest | 10 mg | EUR 393 |
Mouse IgG1-Alexa 488 conjugate (isotype control) |
|||
| 20102-101-A488 | Alpha Diagnostics | 50 Tests | EUR 263 |
AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate] |
|||
| 16890 | AAT Bioquest | 1 mg | EUR 176 |
AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate] |
|||
| 16892 | AAT Bioquest | 1 mg | EUR 176 |
AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin] |
|||
| 23150 | AAT Bioquest | 300 Tests | EUR 306 |
AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin] |
|||
| 23158 | AAT Bioquest | 300 Tests | EUR 306 |
Anti-Hu CD30 Alexa Fluor® 700 |
|||
| A7-455-T100 | ExBio | 100 tests | EUR 269 |
Anti-Hu CD94 Alexa Fluor® 700 |
|||
| A7-727-T100 | ExBio | 100 tests | EUR 269 |
Anti-Hu CD56 Alexa Fluor® 700 |
|||
| A7-789-T100 | ExBio | 100 tests | EUR 269 |
Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
|||
| 43R-ID005AF | Fitzgerald | 500 ug | EUR 338 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
|||
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|||
| 43R-ID022AF | Fitzgerald | 500 ug | EUR 364 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
|||
Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
|||
| 43R-ID028AF | Fitzgerald | 500 ug | EUR 430 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
|||
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|||
| 43R-ID047AF | Fitzgerald | 500 ug | EUR 462 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
|||
Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
|||
| 43R-ID056AF | Fitzgerald | 500 ug | EUR 343 |
|
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
|||
Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
|||
| 43R-ID060AF | Fitzgerald | 300 ug | EUR 425 |
|
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
|||
Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
|||
| 43R-IR016AF | Fitzgerald | 1 mg | EUR 281 |
|
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
|||
GPR12 Antibody |
|||
| 1-CSB-PA008905 | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000 |
|||
GPR12 Antibody |
|||
| 1-CSB-PA009742LA01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
|||
GPR12 Antibody |
|||
| 44910-100ul | SAB | 100ul | EUR 252 |
GPR12 Antibody |
|||
| 44910-50ul | SAB | 50ul | EUR 187 |
GPR12 Antibody |
|||
| DF2721 | Affbiotech | 200ul | EUR 304 |
|
Description: GPR12 antibody detects endogenous levels of total GPR12. |
|||
GPR12 Antibody |
|||
| ABD2721 | Lifescience Market | 100 ug | EUR 438 |
GPR12 antibody |
|||
| 20R-GR041 | Fitzgerald | 50 ug | EUR 656 |
|
Description: Rabbit polyclonal GPR12 antibody |
|||
GPR12 antibody |
|||
| 70R-30940 | Fitzgerald | 100 ug | EUR 327 |
|
Description: Rabbit polyclonal GPR12 antibody |
|||
GPR12 antibody |
|||
| 70R-49804 | Fitzgerald | 100 ul | EUR 244 |
|
Description: Purified Polyclonal GPR12 antibody |
|||
Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated |
|||
| S0005 | Affbiotech | 200ul | EUR 376 |
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated |
|||
| S0006 | Affbiotech | 200ul | EUR 376 |
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0013 | Affbiotech | 200ul | EUR 304 |
Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0014 | Affbiotech | 200ul | EUR 304 |
Mouse pre-microRNA Expression Construct mir-488 |
|||
| MMIR-488-PA-1 | SBI | Bacterial Streak | EUR 684 |
GPR12 |
|||
| RA30050 | Neuromics | 50 ug | EUR 618 |
Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
|||
| Ab8011-001 | GenDepot | 1mg | EUR 334 |
Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647 |
|||
| A6-686-C100 | ExBio | 0.1 mg | EUR 269 |
Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647 |
|||
| A6-712-C100 | ExBio | 0.1 mg | EUR 269 |
Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647 |
|||
| A6-730-C100 | ExBio | 0.1 mg | EUR 269 |
Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647 |
|||
| A6-737-C100 | ExBio | 0.1 mg | EUR 269 |
Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
|||
| 43R-ID012AF | Fitzgerald | 300 ug | EUR 410 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
|||
Gpr12/ Rat Gpr12 ELISA Kit |
|||
| ELI-09764r | Lifescience Market | 96 Tests | EUR 886 |
GPR12 Polyclonal Antibody |
|||
| ES5585-100ul | ELK Biotech | 100ul | EUR 279 |
|
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
|||
GPR12 Polyclonal Antibody |
|||
| ES5585-50ul | ELK Biotech | 50ul | EUR 207 |
|
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
|||
GPR12 Polyclonal Antibody |
|||
| ABP54586-003ml | Abbkine | 0.03ml | EUR 158 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
|||
GPR12 Polyclonal Antibody |
|||
| ABP54586-01ml | Abbkine | 0.1ml | EUR 289 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
|||
GPR12 Polyclonal Antibody |
|||
| ABP54586-02ml | Abbkine | 0.2ml | EUR 414 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
|||
Anti-GPR12 antibody |
|||
| STJ93328 | St John's Laboratory | 200 µl | EUR 197 |
|
Description: Rabbit polyclonal to GPR12. |
|||
GPR12 Conjugated Antibody |
|||
| C44910 | SAB | 100ul | EUR 397 |
GPR12 siRNA |
|||
| 20-abx918427 | Abbexa |
|
|
GPR12 siRNA |
|||
| 20-abx918428 | Abbexa |
|
|
GPR12 siRNA |
|||
| 20-abx902268 | Abbexa |
|
|
GPR12 Antibody, HRP conjugated |
|||
| 1-CSB-PA009742LB01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
|||
GPR12 Antibody, FITC conjugated |
|||
| 1-CSB-PA009742LC01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
|||
GPR12 Antibody, Biotin conjugated |
|||
| 1-CSB-PA009742LD01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
|||
Anti-GPR12/Gpcr12 Antibody |
|||
| A10818 | BosterBio | 100ul | EUR 397 |
|
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human. |
|||
Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
|||
| 50337 | Alpha Diagnostics | 0.5 ml | EUR 225 |
GPR12 Blocking Peptide |
|||
| 20-abx062679 | Abbexa |
|
|
GPR12 Blocking Peptide |
|||
| DF2721-BP | Affbiotech | 1mg | EUR 195 |
Polyclonal GPR12 Antibody (Extracellular Domain) |
|||
| APR16480G | Leading Biology | 0.05mg | EUR 484 |
|
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
|||
Polyclonal GPR12 Antibody (N-Terminus) |
|||
| APR16481G | Leading Biology | 0.05mg | EUR 484 |
|
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications: |
|||
Mouse GPR12 shRNA Plasmid |
|||
| 20-abx970621 | Abbexa |
|
|
Rat GPR12 shRNA Plasmid |
|||
| 20-abx986567 | Abbexa |
|
|
Human GPR12 shRNA Plasmid |
|||
| 20-abx951900 | Abbexa |
|
|
GPR12 ELISA KIT|Human |
|||
| EF005024 | Lifescience Market | 96 Tests | EUR 689 |
GPR12 Recombinant Protein (Rat) |
|||
| RP203285 | ABM | 100 ug | Ask for price |