Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control.
The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.
 Normal Tissue: Adrenal) cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal |
|||
| C1534004-Cy | Biochain | 40 reactions | EUR 540 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 Bovine ADRENAL 50 EA* |
|||
| 57101-2 | Pel-Freez | 50 Each | EUR 756.65 |
 Normal Adrenal Gland Whole tissue lysate) Monkey (Cyno.) Normal Adrenal Gland Whole tissue lysate |
|||
| MCL-1160 | Alpha Diagnostics | 1 mg | EUR 773 |
 Normal Adrenal Gland Whole tissue lysate) Monkey (Rhesus) Normal Adrenal Gland Whole tissue lysate |
|||
| MRL-1267 | Alpha Diagnostics | 1 mg | EUR 773 |
 cDNA from Human Diabetic Tissue: Whole Eye |
|||
| C1236108Dia-10 | Biochain | 10 reactions | EUR 383 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 BAM-12P, Bovine Adrenal Medulla Docosapeptide |
|||
| 5-00748 | CHI Scientific | 4 x 1mg | Ask for price |
 cDNA from Human Adult Normal Tissue: Whole Eye |
|||
| C1234108-10 | Biochain | 10 reactions | EUR 446 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 Normal Tissue: Whole Eye) cDNA from Monkey (Cynomolgus) Normal Tissue: Whole Eye |
|||
| C1534108-Cy | Biochain | 40 reactions | EUR 540 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 Bovine EYE WHOLE 25 EA* |
|||
| 57111-2 | Pel-Freez | 25 EA | EUR 295.55 |
 Bovine KIDNEY WHOLE 2 EA* |
|||
| 57120-2 | Pel-Freez | 2 EA | EUR 174.84 |
 Bovine PITUITARY WHOLE 25 EA* |
|||
| 57133-2 | Pel-Freez | 25 EA | EUR 597.88 |
 Paraffin Tissue Section - Human Adrenal Tumor: Adrenal Pheochromocytoma |
|||
| T2235004-2 | Biochain | 5 slides | EUR 257 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
, Mouse and Rat) Adrenal Dissociation System 9 (Adrenal), Mouse and Rat |
|||
| 4-20209 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Adrenal Dissociation System 2 (Leydig, Adrenal), Mouse and Rat |
|||
| 4-20202 | CHI Scientific | ea | Ask for price |
 Pig ADRENAL 50 EA* |
|||
| 59401-2 | Pel-Freez | 50 EA | EUR 440.19 |
 Rabbit ADRENAL 50 EA |
|||
| 41201-2 | Pel-Freez | 50 EA | EUR 341.23 |
) Rabbit anti Whole Bovine serum antibody (IgG fraction) |
|||
| 40R-RB002 | Fitzgerald | 20 mg | EUR 327 |
|
Description: Affinity purified Rabbit polyclonal Rabbit anti Whole Bovine serum antibody (IgG fraction) |
|||
, Mouse and Rat) Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat |
|||
| 4-20204 | CHI Scientific | ea | Ask for price |
, unlabeled) Rabbit Anti-Bovine IgG (whole IgG from colostrum), unlabeled |
|||
| 80516 | Alpha Diagnostics | 1 ml | EUR 202 |
, unlabeled) Rabbit Anti-Bovine IgG (whole IgG from serum), unlabeled |
|||
| 80517 | Alpha Diagnostics | 1 ml | EUR 202 |
 Frozen Tissue Section - Lupus: Adrenal |
|||
| T1236004Lup | Biochain | 5 slides | EUR 474 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Total Protein from Lupus: Adrenal |
|||
| P1236004Lup | Biochain | 1 mg | EUR 461 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 Rabbit ADRENAL MATURE 50 EA |
|||
| 41301-2 | Pel-Freez | 50 EA | EUR 341.23 |
 Antibody) Histone (Whole) Antibody |
|||
| abx023918-2ml | Abbexa | 2 ml | EUR 509 |
 Rat Whole Heart |
|||
| PC35133 | Neuromics | P0 Rat - Whole Heart X2 | EUR 1341 |
 Mouse Whole Heart |
|||
| PC35135 | Neuromics | P0 Mouse - Whole heart X2 | EUR 1341 |
 Evo? cDNA Kit |
|||
| M1164-100 | Biovision | EUR 294 | |
Evo? cDNA Kit |
|||
| M1164-25 | Biovision | EUR 234 | |
 Novo? cDNA Kit |
|||
| M1165-100 | Biovision | EUR 354 | |
Novo? cDNA Kit |
|||
| M1165-25 | Biovision | EUR 267 | |
 Evo? cDNA Supermix |
|||
| M1168-100 | Biovision | EUR 381 | |
Evo? cDNA Supermix |
|||
| M1168-25 | Biovision | EUR 267 | |
 Novo? cDNA Supermix |
|||
| M1169-100 | Biovision | EUR 441 | |
Novo? cDNA Supermix |
|||
| M1169-25 | Biovision | EUR 289 | |
 cDNA Synthesis SuperMix |
|||
| 20-abx09801420ulSystems | Abbexa |
|
|
 Human adrenal cortex antibody ELISA Kit |
|||
| ELA-E0342h | Lifescience Market | 96 Tests | EUR 824 |
 Frozen Tissue Section - Human Tumor: Adrenal |
|||
| T1235004 | Biochain | 5 slides | EUR 338 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Paraffin Tissue Section - Human Adrenal Tumor |
|||
| T2235004 | Biochain | 5 slides | EUR 257 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Total Protein from Liver Cirrhosis: Adrenal |
|||
| P1236004Lcs | Biochain | 1 mg | EUR 461 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
|||
| C1634310 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
|||
| C1634330 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
|||
| C1634340 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
|||
| C1634350 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
|||
| C1634360 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
|||
| C1634390 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
|||
| C1634370 | Biochain | 40 reactions | EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
) First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
|||
| 20-abx09801620ulSystems | Abbexa |
|
|
) First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
|||
| 20-abx09802120ulSystems | Abbexa |
|
|
 Human adrenal cortex antibody,ACA ELISA Kit |
|||
| CN-03832H1 | ChemNorm | 96T | EUR 452 |
Human adrenal cortex antibody,ACA ELISA Kit |
|||
| CN-03832H2 | ChemNorm | 48T | EUR 302 |
 Human adrenal cortex antibody,ACA ELISA kit |
|||
| E01A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human adrenal cortex antibody,ACA ELISA kit |
|||
| E01A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human adrenal cortex antibody,ACA ELISA kit |
|||
| E01A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Rat adrenal cortex antibody,ACA ELISA kit |
|||
| E02A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rat adrenal cortex antibody,ACA ELISA kit |
|||
| E02A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rat adrenal cortex antibody,ACA ELISA kit |
|||
| E02A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Frozen Tissue Section - Human Adult Normal: Adrenal |
|||
| T1234004 | Biochain | 5 slides | EUR 349 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Frozen Tissue Section - Human Diabetic Tissue: Adrenal |
|||
| T1236004Dia | Biochain | 5 slides | EUR 465 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Paraffin Tissue Section - Human Adult Normal: Adrenal |
|||
| T2234004 | Biochain | 5 slides | EUR 211 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Paraffin Tissue Section - Human Adrenal Tumor: Adenoma |
|||
| T2235004-1 | Biochain | 5 slides | EUR 257 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Paraffin Tissue Section - Human Adrenal Tumor: Myelolipoma |
|||
| T2235004-3 | Biochain | 5 slides | EUR 257 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
|||
 Mouse adrenal cortex antibody,ACA ELISA kit |
|||
| E03A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse adrenal cortex antibody,ACA ELISA kit |
|||
| E03A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse adrenal cortex antibody,ACA ELISA kit |
|||
| E03A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Rabbit adrenal cortex antibody,ACA ELISA kit |
|||
| E04A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rabbit adrenal cortex antibody,ACA ELISA kit |
|||
| E04A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rabbit adrenal cortex antibody,ACA ELISA kit |
|||
| E04A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Total Protein from Human Tumor Tissue: Adrenal |
|||
| P1235004 | Biochain | 1 mg | EUR 461 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 Total Protein from Human Diabetic Tissue: Adrenal |
|||
| P1236004Dia | Biochain | 1 mg | EUR 461 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 Monkey adrenal cortex antibody,ACA ELISA kit |
|||
| E09A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Monkey adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey adrenal cortex antibody,ACA ELISA kit |
|||
| E09A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Monkey adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey adrenal cortex antibody,ACA ELISA kit |
|||
| E09A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Monkey adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Pig adrenal cortex antibody,ACA ELISA kit |
|||
| E07A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Porcine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig adrenal cortex antibody,ACA ELISA kit |
|||
| E07A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Porcine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig adrenal cortex antibody,ACA ELISA kit |
|||
| E07A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Porcine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Dog adrenal cortex antibody,ACA ELISA kit |
|||
| E08A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Canine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Dog adrenal cortex antibody,ACA ELISA kit |
|||
| E08A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Canine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Dog adrenal cortex antibody,ACA ELISA kit |
|||
| E08A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Canine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Goat adrenal cortex antibody,ACA ELISA kit |
|||
| E06A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat adrenal cortex antibody,ACA ELISA kit |
|||
| E06A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat adrenal cortex antibody,ACA ELISA kit |
|||
| E06A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Total RNA from Human Tumor Tissue: Adrenal |
|||
| R1235004-10 | Biochain | 10 ug | EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 Human adrenal cortex antibody, ACA ELISA Kit |
|||
| CSB-E04977h-24T | Cusabio | 1 plate of 24 wells | EUR 165 |
|
Description: Quantitative sandwich ELISA kit for measuring Human adrenal cortex antibody, ACA in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
|||
Human adrenal cortex antibody, ACA ELISA Kit |
|||
| 1-CSB-E04977h | Cusabio |
|
|
|
Description: Quantitative sandwich ELISA kit for measuring Human adrenal cortex antibody, ACA in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
|||
ELISA Kit) Human adrenal cortex antibody(ACA)ELISA Kit |
|||
| GA-E0539HM-48T | GenAsia Biotech | 48T | EUR 289 |
Human adrenal cortex antibody(ACA)ELISA Kit |
|||
| GA-E0539HM-96T | GenAsia Biotech | 96T | EUR 466 |
Human adrenal cortex antibody,ACA ELISA Kit |
|||
| 201-12-0523 | SunredBio | 96 tests | EUR 440 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
|||
 ELISA Kit) Human Adrenal Cortex Antibody (ACA) ELISA Kit |
|||
| abx053206-96tests | Abbexa | 96 tests | EUR 668 |
Human adrenal cortex antibody(ACA)ELISA Kit |
|||
| QY-E01612 | Qayee Biotechnology | 96T | EUR 413 |
) CMV protein (whole cell) |
|||
| 30-AC70 | Fitzgerald | 1 ml | EUR 403 |
|
Description: Purified native CMV protein (whole cell) |
|||
) Monkey IgM (whole molecule) |
|||
| 31R-AI093 | Fitzgerald | 250 µg | EUR 417 |
|
Description: Monkey whole IgM |
|||
 Whole Human Serum antibody |
|||
| 20-1727 | Fitzgerald | 1 L | EUR 30 |
|
Description: Goat polyclonal Whole Human Serum antibody |
|||
Whole Human Serum antibody |
|||
| 20-S1110G000-V0 | Fitzgerald | 10 ml | EUR 133 |
|
Description: Goat polyclonal Whole Human Serum antibody |
|||
 Whole Blood PCR Mix |
|||
| M1143-200 | Biovision | EUR 321 | |
 cDNA from Arteriosclerosis: Aorta |
|||
| C1236012Hd-4 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from hypertension: Artery |
|||
| C1236013Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Arteriosclerosis: Artery |
|||
| C1236013Hd-4 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from hypertension: Vein |
|||
| C1236020Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: Colon |
|||
| C1236090Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from hypertension: Heart |
|||
| C1236122Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: Heart |
|||
| C1236122Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from hypertension: Kidney |
|||
| C1236142Hd-2 | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: Kidney |
|||
| C1236142Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: Liver |
|||
| C1236149Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Asthma: Lung |
|||
| C1236152Ld-1 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Bronchitis: Lung |
|||
| C1236152Ld-2 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Emphysema: Lung |
|||
| C1236152Ld-3 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Pneumonia: Lung |
|||
| C1236152Ld-4 | Biochain | 40 reactions | EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: Lung |
|||
| C1236152Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: Pancreas |
|||
| C1236188Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: Spleen |
|||
| C1236246Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 cDNA from Lupus: stomach |
|||
| C1236248Lup | Biochain | 40 reactions | EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
 OneScriptPlus cDNA Synthesis Kit |
|||
| G235 | ABM | 25 x 20 ul reactions | EUR 97 |
OneScriptPlus cDNA Synthesis Kit |
|||
| G236 | ABM | 100 x 20 ul reactions | EUR 169 |
 OneScriptPlus cDNA Synthesis SuperMix |
|||
| G453 | ABM | 25 x 20 ul reactions | EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
|||
| G454 | ABM | 100 x 20 ul reactions | EUR 169 |
 circRNA cDNA Synthesis Kit |
|||
| G627 | ABM | 25 rxn (20 ul/rxn) | EUR 309 |
 Novo? Transcriptome cDNA Kit |
|||
| M1167-100 | Biovision | EUR 952 | |
Novo? Transcriptome cDNA Kit |
|||
| M1167-25 | Biovision | EUR 441 | |
 cDNA Probe Diluent Solution |
|||
| AR0063 | BosterBio | 5mL | EUR 106 |
 Tetro cDNA Synthesis Kit |
|||
| BIO-65042 | Bioline | 30 Reactions | Ask for price |
Tetro cDNA Synthesis Kit |
|||
| BIO-65043 | Bioline | 100 Reactions | Ask for price |
 SensiFAST cDNA Synthesis Kit |
|||
| BIO-65053 | Bioline | 50 Reactions | Ask for price |
SensiFAST cDNA Synthesis Kit |
|||
| BIO-65053/S | Bioline | Sample | Ask for price |
SensiFAST cDNA Synthesis Kit |
|||
| BIO-65054 | Bioline | 250 Reactions | Ask for price |
 Human eNOS cDNA probe |
|||
| eNOS51-D-2 | Alpha Diagnostics | 2 ug | EUR 445 |
 Plant Tissue cDNA: Arabidopsis |
|||
| PC34-310 | Alpha Diagnostics | 10 rxn | EUR 415 |
 IgG aff pure) Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure |
|||
| HCG14-A | Alpha Diagnostics | 1 mg | EUR 286 |
 Total Protein from Human Adult Normal Tissue: Adrenal |
|||
| P1234004 | Biochain | 1 mg | EUR 356 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 Membrane Protein from Human Adult Normal Tissue: Adrenal |
|||
| P3234004 | Biochain | 0.1 mg | EUR 516 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 Guinea pig adrenal cortex antibody,ACA ELISA kit |
|||
| E05A2028-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Guinea pig adrenal cortex antibody,ACA ELISA kit |
|||
| E05A2028-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Guinea pig adrenal cortex antibody,ACA ELISA kit |
|||
| E05A2028-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Total RNA from Human Adult Normal Tissue: Adrenal |
|||
| R1234004-50 | Biochain | 50 ug | EUR 178 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
, Mouse and Rat) Adrenal Dissociation System 3 (Adrenocortical), Mouse and Rat |
|||
| 4-20203 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Adrenal Dissociation System 7 (Medullary), Mouse and Rat |
|||
| 4-20207 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Adrenal Dissociation System 8 (Cortical), Mouse and Rat |
|||
| 4-20208 | CHI Scientific | ea | Ask for price |
 Mouse FibrOut 5, for adrenal, kidney, pancreas, pituitary |
|||
| 4-20509 | CHI Scientific | 1 ml | Ask for price |
Mouse FibrOut 5, for adrenal, kidney, pancreas, pituitary |
|||
| 4-20510 | CHI Scientific | 5 x 1 ml | Ask for price |
 Rat FibrOut 5, for adrenal, kidney, pancreas, pituitary |
|||
| 4-20535 | CHI Scientific | 1 ml | Ask for price |
Rat FibrOut 5, for adrenal, kidney, pancreas, pituitary |
|||
| 4-20536 | CHI Scientific | 5 x 1 ml | Ask for price |
 Human FibrOut 5, for adrenal, kidney, pancreas, pituitary |
|||
| 4-21554 | CHI Scientific | 1 ml | Ask for price |
Human FibrOut 5, for adrenal, kidney, pancreas, pituitary |
|||
| 4-21555 | CHI Scientific | 5 x 1 ml | Ask for price |
 ELISA kit for Human adrenal cortex antibody,ACA |
|||
| EK0131 | SAB | 96 tests | EUR 712 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Human adrenal cortex antibody,ACA in samples from serum, plasma, tissue homogenates and other biological fluids. |
|||
 QuantiChrom Whole Blood Hb Kit |
|||
| DWHB-250 | BioAssay Systems | 250 | EUR 394 |
|
Description: For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.
Method: OD570nm.
Samples: Whole blood samples.
Species: all.
Procedure: 5 min.
Size: 250 tests.
Detection limit: 0.2 g/dL. |
|||
 Whole Blood DNA Isolation Kit |
|||
| K528-100 | Biovision | EUR 376 | |
 Whole Blood RNA Extraction Kit |
|||
| K1012050 | Biochain | 1 Kit | EUR 333 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
) HeLa Cell Lysate (Whole Cell) |
|||
| LYSATE0001 | Cusabio | 200ug | EUR 150 |
|
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human Hela Whole Cell Lysate |
|||
| LYSATE0023 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human A549 Whole Cell Lysate |
|||
| LYSATE0025 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human Colo320 Whole Cell Lysate |
|||
| LYSATE0026 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human Jurkat Whole Cell Lysate |
|||
| LYSATE0027 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human SW620 Whole Cell Lysate |
|||
| LYSATE0028 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human HepG2 Whole Cell Lysate |
|||
| LYSATE0029 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human K562 Whole Cell Lysate |
|||
| LYSATE0031 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human 293T Whole Cell Lysate |
|||
| LYSATE0032 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human 22RV1 Whole Cell Lysate |
|||
| LYSATE0033 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Human U2OS Whole Cell Lysate |
|||
| LYSATE0034 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Rat RH35 Whole Cell Lysate |
|||
| LYSATE0036 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 Rat PC12 Whole Cell Lysate |
|||
| LYSATE0037 | BosterBio | 200ug | EUR 150 |
|
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
|||
 EpiQuik Whole Cell Extraction Kit |
|||
| OP-0003 | EpiGentek | 100 Extractions | EUR 270.4 |
|
Description: Ask the seller for details |
|||
 Pig WHOLE KIDNEY 10 EA* |
|||
| 59420-2 | Pel-Freez | 10 EA | EUR 237.91 |
 Pig PITUITARY WHOLE 25 EA* |
|||
| 59433-2 | Pel-Freez | 25 EA | EUR 353.19 |
 Rabbit WHOLE EYE 50 EA |
|||
| 41211-2 | Pel-Freez | Each | EUR 318.39 |
 Rabbit KIDNEY WHOLE 50 EA |
|||
| 41220-2 | Pel-Freez | 50 Each | EUR 195.5 |
 Rabbit PITUITARY WHOLE 100 EA* |
|||
| 41233-2 | Pel-Freez | 100 EA | EUR 688.14 |
 CK KIDNEY WHOLE 25 EA* |
|||
| 43020-2 | Pel-Freez | 25 EA | EUR 240.09 |
 CK WHOLE PITUITARY 25 EA* |
|||
| 43033-2 | Pel-Freez | 25 EA | EUR 410.83 |
 Clearview IM Whole Blood test |
|||
| CV506815 | Bionote | 1 box | EUR 106.98 |
|
Description: Please check the datasheet of Clearview IM Whole Blood test before using the test. |
|||
 Mouse Heart Whole tissue lysate |
|||
| MAL-1401 | Alpha Diagnostics | 1 mg | EUR 524 |
 Mouse Lung Whole tissue lysate |
|||
| MAL-1402 | Alpha Diagnostics | 1 mg | EUR 524 |
 Mouse Brain Whole tissue lysate |
|||
| MAL-1403 | Alpha Diagnostics | 1 mg | EUR 524 |
 Mouse Liver Whole tissue lysate |
|||
| MAL-1404 | Alpha Diagnostics | 1 mg | EUR 524 |
 Mouse Kidney Whole tissue lysate |
|||
| MAL-1405 | Alpha Diagnostics | 1 mg | EUR 524 |
 Mouse Spleen Whole tissue lysate |
|||
| MAL-1406 | Alpha Diagnostics | 1 mg | EUR 524 |
 Mouse Thymus Whole tissue lysate |
|||
| MAL-1409 | Alpha Diagnostics | 1 mg | EUR 524 |
 Mouse Bladder Whole tissue lysate |
|||
| MAL-1410 | Alpha Diagnostics | 1 mg | EUR 773 |
A new mathematical model for relative quantification in real-time RT-PCR.
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as well as rapid quantification results. But accurate quantification of nucleic acids requires a reproducible methodology and an adequate mathematical model for data analysis.
This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript. Therefore, a new mathematical model is presented. The relative expression ratio is calculated only from the real-time PCR efficiencies and the crossing point deviation of an unknown sample versus a control. This model needs no calibration curve. Control levels were included in the model to standardise each reaction run with respect to RNA integrity, sample loading and inter-PCR variations. High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model.
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage lambda Red recombinase, which is synthesized under the control of an inducible promoter on an easily curable, low copy number plasmid. To demonstrate the utility of this approach, we generated PCR products by using primers with 36- to 50-nt extensions that are homologous to regions adjacent to the gene to be inactivated and template plasmids carrying antibiotic resistance genes that are flanked by FRT (FLP recognition target) sites. By using the respective PCR products, we made 13 different disruptions of chromosomal genes.
Mutants of the arcB, cyaA, lacZYA, ompR-envZ, phnR, pstB, pstCA, pstS, pstSCAB-phoU, recA, and torSTRCAD genes or operons were isolated as antibiotic-resistant colonies after the introduction into bacteria carrying a Red expression plasmid of synthetic (PCR-generated) DNA. The resistance genes were then eliminated by using a helper plasmid encoding the FLP recombinase which is also easily curable. This procedure should be widely useful, especially in genome analysis of E. coli and other bacteria because the procedure can be done in wild-type cells.
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
BACKGROUND
Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large dynamic range of this methodology, the requirements for a proper internal control gene for normalization have become increasingly stringent. Although housekeeping gene expression has been reported to vary considerably, no systematic survey has properly determined the errors related to the common practice of using only one control gene, nor presented an adequate way of working around this problem.
RESULTS
We outline a robust and innovative strategy to identify the most stably expressed control genes in a given set of tissues, and to determine the minimum number of genes required to calculate a reliable normalization factor. We have evaluated ten housekeeping genes from different abundance and functional classes in various human tissues, and demonstrated that the conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested. The geometric mean of multiple carefully selected housekeeping genes was validated as an accurate normalization factor by analyzing publicly available microarray data.
Analyzing real-time PCR data by the comparative C(T) method.
Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene.
A widely used method to present relative gene expression is the comparative C(T) method also referred to as the 2 (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative C(T) method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.
 AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
|||
| 16891 | AAT Bioquest | 1 mg | EUR 176 |
 AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
|||
| 23153 | AAT Bioquest | 300 Tests | EUR 306 |
 Anti-Hu CD72 Alexa Fluor® 488 |
|||
| A4-310-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Bov CD9 Alexa Fluor® 488 |
|||
| A4-354-C100 | ExBio | 0.1 mg | EUR 269 |
 Endoglin/CD105 Alexa Fluor |
|||
| FC15024 | Neuromics | 100 Tests | EUR 448 |
 Alexa Fluor 488–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
|||
| S0017 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
|||
| S0018 | Affbiotech | 200ul | EUR 304 |
) SAM FCM (Alexa Fluor 647) |
|||
| abx098902-100tests | Abbexa | 100 tests | EUR 1233 |
) Anti-LAMP3 antibody (Alexa-fluor 546) |
|||
| STJ170005 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
|||
) Anti-LAMP3 antibody (Alexa-fluor 647) |
|||
| STJ170006 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
|||
) Anti-IL3RA antibody (Alexa-fluor 546) |
|||
| STJ170010 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
|||
) Anti-IL3RA antibody (Alexa-fluor 647) |
|||
| STJ170011 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
|||
) Anti-CD207 antibody (Alexa-fluor 546) |
|||
| STJ170015 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
|||
) Anti-CD207 antibody (Alexa-fluor 647) |
|||
| STJ170016 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
|||
) Anti-IL7R antibody (Alexa-fluor 546) |
|||
| STJ170021 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R |
|||
) Anti-IL7R antibody (Alexa-fluor 647) |
|||
| STJ170022 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R |
|||
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
|||
| A4-686-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
|||
| A4-712-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
|||
| A4-730-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
|||
| A4-737-C100 | ExBio | 0.1 mg | EUR 269 |
, Alexa Fluor® 488 Conjugated) Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
|||
| Ab8032-001 | GenDepot | 0.5mg | EUR 435 |
 Monoclonal Antibody (104G4) (Alexa Fluor<sup>®</sup> 488)) Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
|||
| M09406 | BosterBio | 100ug | EUR 565 |
|
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
|||
 Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor<sup>®</sup> 488)) Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488) |
|||
| M02316 | BosterBio | 100ug | EUR 580 |
|
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
|||
 Alpha Fluor™ 488 amine |
|||
| 1705 | AAT Bioquest | 1 mg | EUR 306 |
 Alpha Fluor™ 488 Hydroxylamine |
|||
| 1900 | AAT Bioquest | 1 mg | EUR 306 |
 Alpha Fluor™ 488 NHS Ester |
|||
| 1812 | AAT Bioquest | 1 mg | EUR 219 |
 conjugate) Streptavidin-Alexa488 (Alexas fluor 488) conjugate |
|||
| SV-A488-100 | Alpha Diagnostics | 100 tests | EUR 225 |
-Alexa 488 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
|||
| 50336 | Alpha Diagnostics | 0.5 ml | EUR 225 |
 Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
|||
| 1795 | AAT Bioquest | 10 mg | EUR 393 |
) Mouse IgG1-Alexa 488 conjugate (isotype control) |
|||
| 20102-101-A488 | Alpha Diagnostics | 50 Tests | EUR 263 |
 (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
|||
| 43R-ID005AF | Fitzgerald | 500 ug | EUR 338 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
|||
 (Alexa Fluor 594)) Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|||
| 43R-ID022AF | Fitzgerald | 500 ug | EUR 364 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
|||
 (Alexa Fluor 647)) Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
|||
| 43R-ID028AF | Fitzgerald | 500 ug | EUR 430 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
|||
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|||
| 43R-ID047AF | Fitzgerald | 500 ug | EUR 462 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
|||
 (Alexa Fluor 594)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
|||
| 43R-ID056AF | Fitzgerald | 500 ug | EUR 343 |
|
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
|||
 (Alexa Fluor 647)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
|||
| 43R-ID060AF | Fitzgerald | 300 ug | EUR 425 |
|
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
|||
 (Alexa Fluor 594)) Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
|||
| 43R-IR016AF | Fitzgerald | 1 mg | EUR 281 |
|
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
|||
 AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate] |
|||
| 16890 | AAT Bioquest | 1 mg | EUR 176 |
 AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate] |
|||
| 16892 | AAT Bioquest | 1 mg | EUR 176 |
 AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin] |
|||
| 23150 | AAT Bioquest | 300 Tests | EUR 306 |
 AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin] |
|||
| 23158 | AAT Bioquest | 300 Tests | EUR 306 |
 Anti-Hu CD30 Alexa Fluor® 700 |
|||
| A7-455-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD94 Alexa Fluor® 700 |
|||
| A7-727-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD56 Alexa Fluor® 700 |
|||
| A7-789-T100 | ExBio | 100 tests | EUR 269 |
 GPR12 antibody |
|||
| 70R-49804 | Fitzgerald | 100 ul | EUR 244 |
|
Description: Purified Polyclonal GPR12 antibody |
|||
 GPR12 Antibody |
|||
| ABD2721 | Lifescience Market | 100 ug | EUR 438 |
GPR12 antibody |
|||
| 20R-GR041 | Fitzgerald | 50 ug | EUR 656 |
|
Description: Rabbit polyclonal GPR12 antibody |
|||
GPR12 antibody |
|||
| 70R-30940 | Fitzgerald | 100 ug | EUR 327 |
|
Description: Rabbit polyclonal GPR12 antibody |
|||
GPR12 Antibody |
|||
| DF2721 | Affbiotech | 200ul | EUR 304 |
|
Description: GPR12 antibody detects endogenous levels of total GPR12. |
|||
GPR12 Antibody |
|||
| 1-CSB-PA008905 | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000 |
|||
GPR12 Antibody |
|||
| 1-CSB-PA009742LA01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
|||
GPR12 Antibody |
|||
| 44910-100ul | SAB | 100ul | EUR 252 |
GPR12 Antibody |
|||
| 44910-50ul | SAB | 50ul | EUR 187 |
 Alexa Fluor 594–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated |
|||
| S0005 | Affbiotech | 200ul | EUR 376 |
 Alexa Fluor 594–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated |
|||
| S0006 | Affbiotech | 200ul | EUR 376 |
 Alexa Fluor 647–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0013 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor 647–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0014 | Affbiotech | 200ul | EUR 304 |
 GPR12 |
|||
| RA30050 | Neuromics | 50 ug | EUR 618 |
 Mouse pre-microRNA Expression Construct mir-488 |
|||
| MMIR-488-PA-1 | SBI | Bacterial Streak | EUR 684 |
 Gpr12/ Rat Gpr12 ELISA Kit |
|||
| ELI-09764r | Lifescience Market | 96 Tests | EUR 886 |
 (Fab 2) (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
|||
| 43R-ID012AF | Fitzgerald | 300 ug | EUR 410 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
|||
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647 |
|||
| A6-686-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647 |
|||
| A6-712-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647 |
|||
| A6-730-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647 |
|||
| A6-737-C100 | ExBio | 0.1 mg | EUR 269 |
, Alexa Fluor® 594 Conjugated) Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
|||
| Ab8011-001 | GenDepot | 1mg | EUR 334 |
 GPR12 Polyclonal Antibody |
|||
| ABP54586-003ml | Abbkine | 0.03ml | EUR 158 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
|||
GPR12 Polyclonal Antibody |
|||
| ABP54586-01ml | Abbkine | 0.1ml | EUR 289 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
|||
GPR12 Polyclonal Antibody |
|||
| ABP54586-02ml | Abbkine | 0.2ml | EUR 414 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
|||
GPR12 Polyclonal Antibody |
|||
| ES5585-100ul | ELK Biotech | 100ul | EUR 279 |
|
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
|||
GPR12 Polyclonal Antibody |
|||
| ES5585-50ul | ELK Biotech | 50ul | EUR 207 |
|
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
|||
 Anti-GPR12 antibody |
|||
| STJ93328 | St John's Laboratory | 200 µl | EUR 197 |
|
Description: Rabbit polyclonal to GPR12. |
|||
 GPR12 Conjugated Antibody |
|||
| C44910 | SAB | 100ul | EUR 397 |
 GPR12 siRNA |
|||
| 20-abx918427 | Abbexa |
|
|
GPR12 siRNA |
|||
| 20-abx918428 | Abbexa |
|
|
GPR12 siRNA |
|||
| 20-abx902268 | Abbexa |
|
|
 GPR12 Antibody, HRP conjugated |
|||
| 1-CSB-PA009742LB01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
|||
 GPR12 Antibody, FITC conjugated |
|||
| 1-CSB-PA009742LC01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
|||
 GPR12 Antibody, Biotin conjugated |
|||
| 1-CSB-PA009742LD01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
|||
 Anti-GPR12/Gpcr12 Antibody |
|||
| A10818 | BosterBio | 100ul | EUR 397 |
|
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human. |
|||
 GPR12 Blocking Peptide |
|||
| DF2721-BP | Affbiotech | 1mg | EUR 195 |
GPR12 Blocking Peptide |
|||
| 20-abx062679 | Abbexa |
|
|
-Alexa 594 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
|||
| 50337 | Alpha Diagnostics | 0.5 ml | EUR 225 |
) Polyclonal GPR12 Antibody (Extracellular Domain) |
|||
| APR16480G | Leading Biology | 0.05mg | EUR 484 |
|
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
|||
) Polyclonal GPR12 Antibody (N-Terminus) |
|||
| APR16481G | Leading Biology | 0.05mg | EUR 484 |
|
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications: |
|||
 GPR12 ELISA KIT|Human |
|||
| EF005024 | Lifescience Market | 96 Tests | EUR 689 |
) GPR12 Recombinant Protein (Human) |
|||
| RP061035 | ABM | 100 ug | Ask for price |
) GPR12 Recombinant Protein (Rat) |
|||
| RP203285 | ABM | 100 ug | Ask for price |
GPR12 Recombinant Protein (Rat) |
|||
| RP203288 | ABM | 100 ug | Ask for price |
) GPR12 Recombinant Protein (Mouse) |
|||
| RP139421 | ABM | 100 ug | Ask for price |
GPR12 Recombinant Protein (Mouse) |
|||
| RP139424 | ABM | 100 ug | Ask for price |
 Rat GPR12 shRNA Plasmid |
|||
| 20-abx986567 | Abbexa |
|
|
 Human GPR12 shRNA Plasmid |
|||
| 20-abx951900 | Abbexa |
|
|
 Mouse GPR12 shRNA Plasmid |
|||
| 20-abx970621 | Abbexa |
|
|
 Polyclonal GPR12 Antibody - C-terminal region |
|||
| APR16482G | Leading Biology | 0.05mg | EUR 528 |
|
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications: |
|||
 Hepatitis C Virus (HCV) NS5 Genotype-2a ) Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a |
|||
| RP-488 | Alpha Diagnostics | 100 ug | EUR 286 |
 (pORF)) Gpr12 ORF Vector (Rat) (pORF) |
|||
| ORF067763 | ABM | 1.0 ug DNA | EUR 506 |
Gpr12 ORF Vector (Rat) (pORF) |
|||
| ORF067764 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) GPR12 ORF Vector (Human) (pORF) |
|||
| ORF020346 | ABM | 1.0 ug DNA | EUR 405 |
 (pORF)) Gpr12 ORF Vector (Mouse) (pORF) |
|||
| ORF046475 | ABM | 1.0 ug DNA | EUR 506 |
Gpr12 ORF Vector (Mouse) (pORF) |
|||
| ORF046476 | ABM | 1.0 ug DNA | EUR 506 |
 Antibody) G Protein Coupled Receptor 12 (GPR12) Antibody |
|||
| 20-abx338465 | Abbexa |
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody |
|||
| 20-abx324375 | Abbexa |
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody |
|||
| 20-abx147559 | Abbexa |
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody |
|||
| 20-abx009208 | Abbexa |
|
|
 Tide Fluor 2-LL-37 |
|||
| H-8286.0100 | Bachem | 0.1mg | EUR 312 |
|
Description: Sum Formula: C205H340N60O53+dye |
|||
Tide Fluor 2-LL-37 |
|||
| H-8286.0500 | Bachem | 0.5mg | EUR 1017 |
|
Description: Sum Formula: C205H340N60O53+dye |
|||
 Anti-Cytokeratins Alexa Fluor488 |
|||
| A4-108-C025 | ExBio | 0.025 mg | EUR 175 |
Anti-Cytokeratins Alexa Fluor488 |
|||
| A4-108-C100 | ExBio | 0.1 mg | EUR 310 |
 Anti-PSMA Alexa Fluor488 |
|||
| A4-539-C025 | ExBio | 0.025 mg | EUR 227 |
Anti-PSMA Alexa Fluor488 |
|||
| A4-539-C100 | ExBio | 0.1 mg | EUR 414 |
 Anti-FoxP3 Alexa Fluor488 |
|||
| A4-601-C025 | ExBio | 0.025 mg | EUR 201 |
Anti-FoxP3 Alexa Fluor488 |
|||
| A4-601-C100 | ExBio | 0.1 mg | EUR 362 |
 Anti-Phosphotyrosine Alexa Fluor647 |
|||
| A6-263-C025 | ExBio | 0.025 mg | EUR 154 |
Anti-Phosphotyrosine Alexa Fluor647 |
|||
| A6-263-C100 | ExBio | 0.1 mg | EUR 269 |