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Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

Posted on June 29, 2021April 29, 2021 by Leah

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control.
The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.

 

cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal

C1534004-Cy Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Bovine ADRENAL 50 EA*

57101-2 Pel-Freez 50 Each
EUR 756.65

Monkey (Cyno.) Normal Adrenal Gland Whole tissue lysate

MCL-1160 Alpha Diagnostics 1 mg
EUR 773

Monkey (Rhesus) Normal Adrenal Gland Whole tissue lysate

MRL-1267 Alpha Diagnostics 1 mg
EUR 773

cDNA from Human Diabetic Tissue: Whole Eye

C1236108Dia-10 Biochain 10 reactions
EUR 383
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

BAM-12P, Bovine Adrenal Medulla Docosapeptide

5-00748 CHI Scientific 4 x 1mg Ask for price

cDNA from Human Adult Normal Tissue: Whole Eye

C1234108-10 Biochain 10 reactions
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Whole Eye

C1534108-Cy Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Bovine EYE WHOLE 25 EA*

57111-2 Pel-Freez 25 EA
EUR 295.55

Bovine KIDNEY WHOLE 2 EA*

57120-2 Pel-Freez 2 EA
EUR 174.84

Bovine PITUITARY WHOLE 25 EA*

57133-2 Pel-Freez 25 EA
EUR 597.88

Paraffin Tissue Section - Human Adrenal Tumor: Adrenal Pheochromocytoma

T2235004-2 Biochain 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Adrenal Dissociation System 9 (Adrenal), Mouse and Rat

4-20209 CHI Scientific ea Ask for price

Adrenal Dissociation System 2 (Leydig, Adrenal), Mouse and Rat

4-20202 CHI Scientific ea Ask for price

Pig ADRENAL 50 EA*

59401-2 Pel-Freez 50 EA
EUR 440.19

Rabbit ADRENAL 50 EA

41201-2 Pel-Freez 50 EA
EUR 341.23

Rabbit anti Whole Bovine serum antibody (IgG fraction)

40R-RB002 Fitzgerald 20 mg
EUR 327
Description: Affinity purified Rabbit polyclonal Rabbit anti Whole Bovine serum antibody (IgG fraction)

Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat

4-20204 CHI Scientific ea Ask for price

Rabbit Anti-Bovine IgG (whole IgG from colostrum), unlabeled

80516 Alpha Diagnostics 1 ml
EUR 202

Rabbit Anti-Bovine IgG (whole IgG from serum), unlabeled

80517 Alpha Diagnostics 1 ml
EUR 202

Frozen Tissue Section - Lupus: Adrenal

T1236004Lup Biochain 5 slides
EUR 474
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein from Lupus: Adrenal

P1236004Lup Biochain 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Rabbit ADRENAL MATURE 50 EA

41301-2 Pel-Freez 50 EA
EUR 341.23

Histone (Whole) Antibody

abx023918-2ml Abbexa 2 ml
EUR 509

Rat Whole Heart

PC35133 Neuromics P0 Rat - Whole Heart X2
EUR 1341

Mouse Whole Heart

PC35135 Neuromics P0 Mouse - Whole heart X2
EUR 1341

Evo? cDNA Kit

M1164-100 Biovision
EUR 294

Evo? cDNA Kit

M1164-25 Biovision
EUR 234

Novo? cDNA Kit

M1165-100 Biovision
EUR 354

Novo? cDNA Kit

M1165-25 Biovision
EUR 267

Evo? cDNA Supermix

M1168-100 Biovision
EUR 381

Evo? cDNA Supermix

M1168-25 Biovision
EUR 267

Novo? cDNA Supermix

M1169-100 Biovision
EUR 441

Novo? cDNA Supermix

M1169-25 Biovision
EUR 289

cDNA Synthesis SuperMix

20-abx09801420ulSystems Abbexa
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Human adrenal cortex antibody ELISA Kit

ELA-E0342h Lifescience Market 96 Tests
EUR 824

Frozen Tissue Section - Human Tumor: Adrenal

T1235004 Biochain 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adrenal Tumor

T2235004 Biochain 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein from Liver Cirrhosis: Adrenal

P1236004Lcs Biochain 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems Abbexa
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems Abbexa
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Human adrenal cortex antibody,ACA ELISA Kit

CN-03832H1 ChemNorm 96T
EUR 452

Human adrenal cortex antibody,ACA ELISA Kit

CN-03832H2 ChemNorm 48T
EUR 302

Human adrenal cortex antibody,ACA ELISA kit

E01A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human adrenal cortex antibody,ACA ELISA kit

E01A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human adrenal cortex antibody,ACA ELISA kit

E01A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat adrenal cortex antibody,ACA ELISA kit

E02A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat adrenal cortex antibody,ACA ELISA kit

E02A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat adrenal cortex antibody,ACA ELISA kit

E02A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Frozen Tissue Section - Human Adult Normal: Adrenal

T1234004 Biochain 5 slides
EUR 349
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Diabetic Tissue: Adrenal

T1236004Dia Biochain 5 slides
EUR 465
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Adrenal

T2234004 Biochain 5 slides
EUR 211
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adrenal Tumor: Adenoma

T2235004-1 Biochain 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adrenal Tumor: Myelolipoma

T2235004-3 Biochain 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Mouse adrenal cortex antibody,ACA ELISA kit

E03A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse adrenal cortex antibody,ACA ELISA kit

E03A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse adrenal cortex antibody,ACA ELISA kit

E03A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit adrenal cortex antibody,ACA ELISA kit

E04A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit adrenal cortex antibody,ACA ELISA kit

E04A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit adrenal cortex antibody,ACA ELISA kit

E04A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total Protein from Human Tumor Tissue: Adrenal

P1235004 Biochain 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Adrenal

P1236004Dia Biochain 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Monkey adrenal cortex antibody,ACA ELISA kit

E09A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey adrenal cortex antibody,ACA ELISA kit

E09A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey adrenal cortex antibody,ACA ELISA kit

E09A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig adrenal cortex antibody,ACA ELISA kit

E07A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig adrenal cortex antibody,ACA ELISA kit

E07A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig adrenal cortex antibody,ACA ELISA kit

E07A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog adrenal cortex antibody,ACA ELISA kit

E08A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog adrenal cortex antibody,ACA ELISA kit

E08A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog adrenal cortex antibody,ACA ELISA kit

E08A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat adrenal cortex antibody,ACA ELISA kit

E06A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat adrenal cortex antibody,ACA ELISA kit

E06A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat adrenal cortex antibody,ACA ELISA kit

E06A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA from Human Tumor Tissue: Adrenal

R1235004-10 Biochain 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human adrenal cortex antibody, ACA ELISA Kit

CSB-E04977h-24T Cusabio 1 plate of 24 wells
EUR 165
Description: Quantitative sandwich ELISA kit for measuring Human adrenal cortex antibody, ACA in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human adrenal cortex antibody, ACA ELISA Kit

1-CSB-E04977h Cusabio
  • EUR 900.00
  • EUR 4238.00
  • EUR 2256.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human adrenal cortex antibody, ACA in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human adrenal cortex antibody(ACA)ELISA Kit

GA-E0539HM-48T GenAsia Biotech 48T
EUR 289

Human adrenal cortex antibody(ACA)ELISA Kit

GA-E0539HM-96T GenAsia Biotech 96T
EUR 466

Human adrenal cortex antibody,ACA ELISA Kit

201-12-0523 SunredBio 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Adrenal Cortex Antibody (ACA) ELISA Kit

abx053206-96tests Abbexa 96 tests
EUR 668

Human adrenal cortex antibody(ACA)ELISA Kit

QY-E01612 Qayee Biotechnology 96T
EUR 413

CMV protein (whole cell)

30-AC70 Fitzgerald 1 ml
EUR 403
Description: Purified native CMV protein (whole cell)

Monkey IgM (whole molecule)

31R-AI093 Fitzgerald 250 µg
EUR 417
Description: Monkey whole IgM

Whole Human Serum antibody

20-1727 Fitzgerald 1 L
EUR 30
Description: Goat polyclonal Whole Human Serum antibody

Whole Human Serum antibody

20-S1110G000-V0 Fitzgerald 10 ml
EUR 133
Description: Goat polyclonal Whole Human Serum antibody

Whole Blood PCR Mix

M1143-200 Biovision
EUR 321

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

OneScriptPlus cDNA Synthesis Kit

G235 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 ABM 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 ABM 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 ABM 25 rxn (20 ul/rxn)
EUR 309

Novo? Transcriptome cDNA Kit

M1167-100 Biovision
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25 Biovision
EUR 441

cDNA Probe Diluent Solution

AR0063 BosterBio 5mL
EUR 106

Tetro cDNA Synthesis Kit

BIO-65042 Bioline 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 Bioline 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 Bioline 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Bioline Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 Bioline 250 Reactions Ask for price

Human eNOS cDNA probe

eNOS51-D-2 Alpha Diagnostics 2 ug
EUR 445

Plant Tissue cDNA: Arabidopsis

PC34-310 Alpha Diagnostics 10 rxn
EUR 415

Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure

HCG14-A Alpha Diagnostics 1 mg
EUR 286

Total Protein from Human Adult Normal Tissue: Adrenal

P1234004 Biochain 1 mg
EUR 356
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Adult Normal Tissue: Adrenal

P3234004 Biochain 0.1 mg
EUR 516
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Guinea pig adrenal cortex antibody,ACA ELISA kit

E05A2028-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Guinea pig adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig adrenal cortex antibody,ACA ELISA kit

E05A2028-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Guinea pig adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig adrenal cortex antibody,ACA ELISA kit

E05A2028-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Guinea pig adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA from Human Adult Normal Tissue: Adrenal

R1234004-50 Biochain 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Adrenal Dissociation System 3 (Adrenocortical), Mouse and Rat

4-20203 CHI Scientific ea Ask for price

Adrenal Dissociation System 7 (Medullary), Mouse and Rat

4-20207 CHI Scientific ea Ask for price

Adrenal Dissociation System 8 (Cortical), Mouse and Rat

4-20208 CHI Scientific ea Ask for price

Mouse FibrOut 5, for adrenal, kidney, pancreas, pituitary

4-20509 CHI Scientific 1 ml Ask for price

Mouse FibrOut 5, for adrenal, kidney, pancreas, pituitary

4-20510 CHI Scientific 5 x 1 ml Ask for price

Rat FibrOut 5, for adrenal, kidney, pancreas, pituitary

4-20535 CHI Scientific 1 ml Ask for price

Rat FibrOut 5, for adrenal, kidney, pancreas, pituitary

4-20536 CHI Scientific 5 x 1 ml Ask for price

Human FibrOut 5, for adrenal, kidney, pancreas, pituitary

4-21554 CHI Scientific 1 ml Ask for price

Human FibrOut 5, for adrenal, kidney, pancreas, pituitary

4-21555 CHI Scientific 5 x 1 ml Ask for price

ELISA kit for Human adrenal cortex antibody,ACA

EK0131 SAB 96 tests
EUR 712
Description: Enzyme-linked immunosorbent assay kit for quantification of Human adrenal cortex antibody,ACA in samples from serum, plasma, tissue homogenates and other biological fluids.

QuantiChrom Whole Blood Hb Kit

DWHB-250 BioAssay Systems 250
EUR 394
Description: For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism. Method: OD570nm. Samples: Whole blood samples. Species: all. Procedure: 5 min. Size: 250 tests. Detection limit: 0.2 g/dL.

Whole Blood DNA Isolation Kit

K528-100 Biovision
EUR 376

Whole Blood RNA Extraction Kit

K1012050 Biochain 1 Kit
EUR 333
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

HeLa Cell Lysate (Whole Cell)

LYSATE0001 Cusabio 200ug
EUR 150
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Hela Whole Cell Lysate

LYSATE0023 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate

LYSATE0025 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Colo320 Whole Cell Lysate

LYSATE0026 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Jurkat Whole Cell Lysate

LYSATE0027 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human SW620 Whole Cell Lysate

LYSATE0028 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate

LYSATE0029 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate

LYSATE0031 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 293T Whole Cell Lysate

LYSATE0032 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate

LYSATE0033 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate

LYSATE0034 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat RH35 Whole Cell Lysate

LYSATE0036 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate

LYSATE0037 BosterBio 200ug
EUR 150
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

EpiQuik Whole Cell Extraction Kit

OP-0003 EpiGentek 100 Extractions
EUR 270.4
Description: Ask the seller for details

Pig WHOLE KIDNEY 10 EA*

59420-2 Pel-Freez 10 EA
EUR 237.91

Pig PITUITARY WHOLE 25 EA*

59433-2 Pel-Freez 25 EA
EUR 353.19

Rabbit WHOLE EYE 50 EA

41211-2 Pel-Freez Each
EUR 318.39

Rabbit KIDNEY WHOLE 50 EA

41220-2 Pel-Freez 50 Each
EUR 195.5

Rabbit PITUITARY WHOLE 100 EA*

41233-2 Pel-Freez 100 EA
EUR 688.14

CK KIDNEY WHOLE 25 EA*

43020-2 Pel-Freez 25 EA
EUR 240.09

CK WHOLE PITUITARY 25 EA*

43033-2 Pel-Freez 25 EA
EUR 410.83

Clearview IM Whole Blood test

CV506815 Bionote 1 box
EUR 106.98
Description: Please check the datasheet of Clearview IM Whole Blood test before using the test.

Mouse Heart Whole tissue lysate

MAL-1401 Alpha Diagnostics 1 mg
EUR 524

Mouse Lung Whole tissue lysate

MAL-1402 Alpha Diagnostics 1 mg
EUR 524

Mouse Brain Whole tissue lysate

MAL-1403 Alpha Diagnostics 1 mg
EUR 524

Mouse Liver Whole tissue lysate

MAL-1404 Alpha Diagnostics 1 mg
EUR 524

Mouse Kidney Whole tissue lysate

MAL-1405 Alpha Diagnostics 1 mg
EUR 524

Mouse Spleen Whole tissue lysate

MAL-1406 Alpha Diagnostics 1 mg
EUR 524

Mouse Thymus Whole tissue lysate

MAL-1409 Alpha Diagnostics 1 mg
EUR 524

Mouse Bladder Whole tissue lysate

MAL-1410 Alpha Diagnostics 1 mg
EUR 773
inqua-saccom
inqua-saccom

A new mathematical model for relative quantification in real-time RT-PCR.

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as well as rapid quantification results. But accurate quantification of nucleic acids requires a reproducible methodology and an adequate mathematical model for data analysis.
This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript. Therefore, a new mathematical model is presented. The relative expression ratio is calculated only from the real-time PCR efficiencies and the crossing point deviation of an unknown sample versus a control. This model needs no calibration curve. Control levels were included in the model to standardise each reaction run with respect to RNA integrity, sample loading and inter-PCR variations. High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model.

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage lambda Red recombinase, which is synthesized under the control of an inducible promoter on an easily curable, low copy number plasmid. To demonstrate the utility of this approach, we generated PCR products by using primers with 36- to 50-nt extensions that are homologous to regions adjacent to the gene to be inactivated and template plasmids carrying antibiotic resistance genes that are flanked by FRT (FLP recognition target) sites. By using the respective PCR products, we made 13 different disruptions of chromosomal genes.
Mutants of the arcB, cyaA, lacZYA, ompR-envZ, phnR, pstB, pstCA, pstS, pstSCAB-phoU, recA, and torSTRCAD genes or operons were isolated as antibiotic-resistant colonies after the introduction into bacteria carrying a Red expression plasmid of synthetic (PCR-generated) DNA. The resistance genes were then eliminated by using a helper plasmid encoding the FLP recombinase which is also easily curable. This procedure should be widely useful, especially in genome analysis of E. coli and other bacteria because the procedure can be done in wild-type cells.

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

BACKGROUND
Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large dynamic range of this methodology, the requirements for a proper internal control gene for normalization have become increasingly stringent. Although housekeeping gene expression has been reported to vary considerably, no systematic survey has properly determined the errors related to the common practice of using only one control gene, nor presented an adequate way of working around this problem.
RESULTS
We outline a robust and innovative strategy to identify the most stably expressed control genes in a given set of tissues, and to determine the minimum number of genes required to calculate a reliable normalization factor. We have evaluated ten housekeeping genes from different abundance and functional classes in various human tissues, and demonstrated that the conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested. The geometric mean of multiple carefully selected housekeeping genes was validated as an accurate normalization factor by analyzing publicly available microarray data.

Analyzing real-time PCR data by the comparative C(T) method.

Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene.
A widely used method to present relative gene expression is the comparative C(T) method also referred to as the 2 (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative C(T) method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.

 

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 AAT Bioquest 1 mg
EUR 176

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 AAT Bioquest 300 Tests
EUR 306

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 ExBio 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 ExBio 0.1 mg
EUR 269

Endoglin/CD105 Alexa Fluor

FC15024 Neuromics 100 Tests
EUR 448

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

S0017 Affbiotech 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

S0018 Affbiotech 200ul
EUR 304

SAM FCM (Alexa Fluor 647)

abx098902-100tests Abbexa 100 tests
EUR 1233

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 St John's Laboratory 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)

STJ170006 St John's Laboratory 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 St John's Laboratory 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)

STJ170011 St John's Laboratory 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 St John's Laboratory 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 St John's Laboratory 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 St John's Laboratory 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 St John's Laboratory 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 ExBio 0.1 mg
EUR 269

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 GenDepot 0.5mg
EUR 435

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 BosterBio 100ug
EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 BosterBio 100ug
EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Alpha Fluor™ 488 amine

1705 AAT Bioquest 1 mg
EUR 306

Alpha Fluor™ 488 Hydroxylamine

1900 AAT Bioquest 1 mg
EUR 306

Alpha Fluor™ 488 NHS Ester

1812 AAT Bioquest 1 mg
EUR 219

Streptavidin-Alexa488 (Alexas fluor 488) conjugate

SV-A488-100 Alpha Diagnostics 100 tests
EUR 225

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 Alpha Diagnostics 0.5 ml
EUR 225

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

1795 AAT Bioquest 10 mg
EUR 393

Mouse IgG1-Alexa 488 conjugate (isotype control)

20102-101-A488 Alpha Diagnostics 50 Tests
EUR 263

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF Fitzgerald 500 ug
EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF Fitzgerald 500 ug
EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

43R-ID028AF Fitzgerald 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF Fitzgerald 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-ID056AF Fitzgerald 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

43R-ID060AF Fitzgerald 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF Fitzgerald 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

16890 AAT Bioquest 1 mg
EUR 176

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 AAT Bioquest 1 mg
EUR 176

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

23150 AAT Bioquest 300 Tests
EUR 306

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 AAT Bioquest 300 Tests
EUR 306

Anti-Hu CD30 Alexa Fluor® 700

A7-455-T100 ExBio 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700

A7-727-T100 ExBio 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700

A7-789-T100 ExBio 100 tests
EUR 269

GPR12 antibody

70R-49804 Fitzgerald 100 ul
EUR 244
Description: Purified Polyclonal GPR12 antibody

GPR12 Antibody

ABD2721 Lifescience Market 100 ug
EUR 438

GPR12 antibody

20R-GR041 Fitzgerald 50 ug
EUR 656
Description: Rabbit polyclonal GPR12 antibody

GPR12 antibody

70R-30940 Fitzgerald 100 ug
EUR 327
Description: Rabbit polyclonal GPR12 antibody

GPR12 Antibody

DF2721 Affbiotech 200ul
EUR 304
Description: GPR12 antibody detects endogenous levels of total GPR12.

GPR12 Antibody

1-CSB-PA008905 Cusabio
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 Antibody

1-CSB-PA009742LA01HU Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GPR12 Antibody

44910-100ul SAB 100ul
EUR 252

GPR12 Antibody

44910-50ul SAB 50ul
EUR 187

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated

S0005 Affbiotech 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

S0006 Affbiotech 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

S0013 Affbiotech 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

S0014 Affbiotech 200ul
EUR 304

GPR12

RA30050 Neuromics 50 ug
EUR 618

Mouse pre-microRNA Expression Construct mir-488

MMIR-488-PA-1 SBI Bacterial Streak
EUR 684

Gpr12/ Rat Gpr12 ELISA Kit

ELI-09764r Lifescience Market 96 Tests
EUR 886

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

43R-ID012AF Fitzgerald 300 ug
EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

A6-686-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

A6-712-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

A6-730-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

A6-737-C100 ExBio 0.1 mg
EUR 269

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 GenDepot 1mg
EUR 334

GPR12 Polyclonal Antibody

ABP54586-003ml Abbkine 0.03ml
EUR 158
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-01ml Abbkine 0.1ml
EUR 289
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-02ml Abbkine 0.2ml
EUR 414
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ES5585-100ul ELK Biotech 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

ES5585-50ul ELK Biotech 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

Anti-GPR12 antibody

STJ93328 St John's Laboratory 200 µl
EUR 197
Description: Rabbit polyclonal to GPR12.

GPR12 Conjugated Antibody

C44910 SAB 100ul
EUR 397

GPR12 siRNA

20-abx918427 Abbexa
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GPR12 siRNA

20-abx918428 Abbexa
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GPR12 siRNA

20-abx902268 Abbexa
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GPR12 Antibody, HRP conjugated

1-CSB-PA009742LB01HU Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated

1-CSB-PA009742LC01HU Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated

1-CSB-PA009742LD01HU Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Anti-GPR12/Gpcr12 Antibody

A10818 BosterBio 100ul
EUR 397
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human.

GPR12 Blocking Peptide

DF2721-BP Affbiotech 1mg
EUR 195

GPR12 Blocking Peptide

20-abx062679 Abbexa
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

50337 Alpha Diagnostics 0.5 ml
EUR 225

Polyclonal GPR12 Antibody (Extracellular Domain)

APR16480G Leading Biology 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (N-Terminus)

APR16481G Leading Biology 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

GPR12 ELISA KIT|Human

EF005024 Lifescience Market 96 Tests
EUR 689

GPR12 Recombinant Protein (Human)

RP061035 ABM 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203285 ABM 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203288 ABM 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139421 ABM 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139424 ABM 100 ug Ask for price

Rat GPR12 shRNA Plasmid

20-abx986567 Abbexa
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human GPR12 shRNA Plasmid

20-abx951900 Abbexa
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse GPR12 shRNA Plasmid

20-abx970621 Abbexa
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Polyclonal GPR12 Antibody - C-terminal region

APR16482G Leading Biology 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:

Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a 

RP-488 Alpha Diagnostics 100 ug
EUR 286

Gpr12 ORF Vector (Rat) (pORF)

ORF067763 ABM 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Rat) (pORF)

ORF067764 ABM 1.0 ug DNA
EUR 506

GPR12 ORF Vector (Human) (pORF)

ORF020346 ABM 1.0 ug DNA
EUR 405

Gpr12 ORF Vector (Mouse) (pORF)

ORF046475 ABM 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Mouse) (pORF)

ORF046476 ABM 1.0 ug DNA
EUR 506

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx338465 Abbexa
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx324375 Abbexa
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx147559 Abbexa
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx009208 Abbexa
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Tide Fluor 2-LL-37

H-8286.0100 Bachem 0.1mg
EUR 312
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37

H-8286.0500 Bachem 0.5mg
EUR 1017
Description: Sum Formula: C205H340N60O53+dye

Anti-Cytokeratins Alexa Fluor488

A4-108-C025 ExBio 0.025 mg
EUR 175

Anti-Cytokeratins Alexa Fluor488

A4-108-C100 ExBio 0.1 mg
EUR 310

Anti-PSMA Alexa Fluor488

A4-539-C025 ExBio 0.025 mg
EUR 227

Anti-PSMA Alexa Fluor488

A4-539-C100 ExBio 0.1 mg
EUR 414

Anti-FoxP3 Alexa Fluor488

A4-601-C025 ExBio 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor488

A4-601-C100 ExBio 0.1 mg
EUR 362

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C025 ExBio 0.025 mg
EUR 154

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C100 ExBio 0.1 mg
EUR 269

 

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    Recent Posts

    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
    • Engineering hybrid genes without the use of restriction enzymes: gene splicing
    • Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.
    • Supervised risk predictor of breast cancer based on intrinsic subtypes.
    • Aqua EZ Clean
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