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Month: June 2021

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

Posted on June 29, 2021April 29, 2021 by Leah

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript… Continue reading

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Engineering hybrid genes without the use of restriction enzymes: gene splicing

Posted on June 26, 2021April 29, 2021 by Leah

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene splicing by overlap extension is a new approach for recombining DNA molecules at precise junctions irrespective of nucleotide sequences at the recombination site and without the use of restriction endonucleases or ligase. Fragments from the genes that are to be… Continue reading

Recent Posts

  • Compare rec. lab reagents for research
  • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
  • Engineering hybrid genes without the use of restriction enzymes: gene splicing
  • Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.
  • Supervised risk predictor of breast cancer based on intrinsic subtypes.

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    Categories

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    Recent Posts

    • Compare rec. lab reagents for research
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
    • Engineering hybrid genes without the use of restriction enzymes: gene splicing
    • Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.
    • Supervised risk predictor of breast cancer based on intrinsic subtypes.
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